التفاصيل البيبلوغرافية
| العنوان: |
SCIENCE CHINA Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production |
| المؤلفون: |
Lai Shujuan, Yun Zhang, Shuwen Liu, Yong Liang, Xiuling Shang, Chai Xin, Wen Tingyi |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
http://www.im.ac.cn/UserFiles/File/2012/2012-07/Science%20China.pdfTest. |
| سنة النشر: |
2012 |
| المجموعة: |
CiteSeerX |
| الوصف: |
L-Serine plays a critical role as a building block for cell growth, and thus it is difficult to achieve the direct fermentation of L-serine from glucose. In this study, Corynebacterium glutamicum ATCC 13032 was engineered de novo by blocking and attenuating the conversion of L-serine to pyruvate and glycine, releasing the feedback inhibition by L-serine to 3-phosphoglycerate dehydrogenase (PGDH), in combination with the co-expression of 3-phosphoglycerate kinase (PGK) and feedback-resistant PGDH (PGDH r ). The resulting strain, SER-8, exhibited a lower specific growth rate and significant differences in L-serine levels from Phase I to Phase V as determined for fed-batch fermentation. The intracellular L-serine pool reached (14.22±1.41) μmol g CDM 1 , which was higher than glycine pool, contrary to fermentation with the wild-type strain. Furthermore, metabolic flux analysis demonstrated that the over-expression of PGK directed the flux of the pentose phosphate pathway (PPP) towards the glycolysis pathway (EMP), and the expression of PGDH r improved the L-serine biosynthesis pathway. In addition, the flux from L-serine to glycine dropped by 24%, indicating that the deletion of the activator GlyR resulted in down-regulation of serine hydroxymethyltransferase (SHMT) expression. Taken together, our findings imply that L-serine pool management is fundamental for sustaining the viability of C. glutamicum, and improvement of C 1 units generation by introducing the glycine cleavage system (GCV) to degrade the excessive glycine is a promising target for L-serine production in C. glutamicum. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1078.9733Test; http://www.im.ac.cn/UserFiles/File/2012/2012-07/Science%20China.pdfTest |
| الإتاحة: |
http://www.im.ac.cn/UserFiles/File/2012/2012-07/Science%20China.pdfTest |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.AFFD655A |
| قاعدة البيانات: |
BASE |
