المؤلفون: Artem Blagodatski, Vera Batrak, Sabine Schmidl, Ulrike Schoetz, Olph B. Caldwell, Jean-marie Buerstedde

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/44/ce/PLoS_Genet_2009_Jan_9_5(1)_e1000332.tar.gz

مصطلحات موضوعية: These authors contributed equally to this work

الوصف: Hypermutation of the immunoglobulin (Ig) genes requires Activation Induced cytidine Deaminase (AID) and transcription, but it remains unclear why other transcribed genes of B cells do not mutate. We describe a reporter transgene crippled by hypermutation when inserted into or near the Ig light chain (IgL) locus of the DT40 B cell line yet stably expressed when inserted into other chromosomal positions. Step-wise deletions of the IgL locus revealed that a sequence extending for 9.8 kilobases downstream of the IgL transcription start site confers the hypermutation activity. This sequence, named DIVAC for diversification activator, efficiently activates hypermutation when inserted at non-Ig loci. The results significantly extend previously reported findings on AID-mediated gene diversification. They show by both deletion and insertion analyses that

وصف الملف: application/zip

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.358.6977

المؤلفون: Hiroshi Arakawa, Hiroaki Kudo, Vera Batrak, Olph B. Caldwell, Michael A. Rieger, Joachim W. Ellwart, Jean-marie Buerstedde

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: http://nar.oxfordjournals.org/content/36/1/e1.full-text-lowres.pdfTest.

الوصف: in the B cell line DT40

وصف الملف: application/pdf

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.554.8280; http://nar.oxfordjournals.org/content/36/1/e1.full-text-lowres.pdfTest

الإتاحة: http://nar.oxfordjournals.org/content/36/1/e1.full-text-lowres.pdfTest

المؤلفون: Hiroshi Arakawa, Hiroaki Kudo, Vera Batrak, Olph B. Caldwell, Michael A. Rieger, Joachim W. Ellwart, Jean-marie Buerstedde

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: http://nar.oxfordjournals.org/content/early/2007/12/11/nar.gkm616.full-text-lowres.pdfTest.

الوصف: in the B cell line DT40

وصف الملف: application/pdf

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.494.8534; http://nar.oxfordjournals.org/content/early/2007/12/11/nar.gkm616.full-text-lowres.pdfTest

الإتاحة: http://nar.oxfordjournals.org/content/early/2007/12/11/nar.gkm616.full-text-lowres.pdfTest

المؤلفون: Hiroshi Arakawa, Hiroaki Kudo, Vera Batrak, Olph B. Caldwell, Michael A. Rieger, Joachim W. Ellwart, Jean-marie Buerstedde

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: http://www.helmholtz-muenchen.de/fileadmin/ISF/PDF/Haematopoese/publications/Arakawa_et_al.__NAR_2007.pdfTest.

الوصف: in the B cell line DT40

وصف الملف: application/pdf

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.482.8177; http://www.helmholtz-muenchen.de/fileadmin/ISF/PDF/Haematopoese/publications/Arakawa_et_al.__NAR_2007.pdfTest

الإتاحة: http://www.helmholtz-muenchen.de/fileadmin/ISF/PDF/Haematopoese/publications/Arakawa_et_al.__NAR_2007.pdfTest

المؤلفون: Hiroshi Arakawa, Hiroaki Kudo, Vera Batrak, Olph B. Caldwell, Michael A. Rieger, Joachim W. Ellwart, Jean-marie Buerstedde

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/96/0a/Nucleic_Acids_Res_2008_Jan_36(1)_e1.tar.gz

الوصف: in the B cell line DT40

وصف الملف: application/zip

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.282.2871