التفاصيل البيبلوغرافية
| العنوان: |
EXPERIMENTAL STUDY Somatostatin analogues stimulate p27 expression and inhibit the MAP kinase pathway in pituitary tumours |
| المؤلفون: |
Erika Hubina, Ra M Nanzer, Matthew R Hanson, Enrica Ciccarelli, Marco Losa, Daniela Gaia, Mauro Papotti, Maria Rosaria Terreni, Sahira Khalaf, Suzanne Jordan, Sándor Czirják, Zoltán Hanzély, György M Nagy, Miklós Igóth, Ashley B Grossman, Márta Korbonits |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
http://www.eje-online.org/content/155/2/371.full.pdfTest. |
| المجموعة: |
CiteSeerX |
| الوصف: |
Objectives: Somatostatin (SST) analogues play an important role in the medical management of somatotroph pituitary adenomas and new agonists have the potential to be effective in a wider group of pituitary and other tumours. The anti-proliferative effect of SST occurs through multiple mechanisms, one of which is cell-cycle arrest, where p27, a cyclin-dependent kinase inhibitor, is an important regulator. We hypothesised that SST may upregulate p27 protein levels and downregulate the MAP kinase pathway in these tumours. Methods: Human pituitary adenoma cells and rat pituitary cell line (GH3) were cultured and treated in vitro with octreotide and the broad-spectrum SST agonist SOM230 (pasireotide). Immunoblotting for p27 and phospho-ERK (pERK) was performed and proliferation assessed by [ 3 H]-thymidine incorporation. Histological samples from acromegalic patients treated with octreotide before surgery were immunostained for p27 and compared to samples from untreated patients matched for sex, age, tumour size, extension and invasiveness. Results: We detected upregulation of p27 protein levels with SST analogue treatment in vitro in human |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.317.5327Test; http://www.eje-online.org/content/155/2/371.full.pdfTest |
| الإتاحة: |
http://www.eje-online.org/content/155/2/371.full.pdfTest |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.3736D43 |
| قاعدة البيانات: |
BASE |
