| الوصف: |
Staphylococcus aureus, but some other staphylococci species also, is capable to produce toxins and some extracelular proteins like: enterotoxins, coagulasis, A protein, hemolysins, fibrinolysins, nucleases and staphylochinoses (2). It is well known that major factors of pathogenity of species belonging to Staphylococcus genus are represented by enterotoxins, hemolysins and coagulasis (6, 7, 8). Data from specialized literature specify that regardless of specie, the majority of Staphylococcus strains are not capable to synthesize al pathogenity factors. Considering this characteristic of species belonging to Staphylococcus genus, this research try to establish a correlation between the principal factors of pathogenity represented by coagulasis, hemolysins and enterotoxins. Materials and methods The research was conducted on 186 staphylococci strains, isolated from raw milk and milk products. The isolation and identification of strains belonging to Staphylococcus genus was conducted in accord with the methodology statedby EN ISO 6888 procedure (11). The capacity of coagulasis secretion was determined by in tubs method toward rabbit serum, hemolysins secretion was determined on 10 % sheep blood nutritive agar. The identification of Staphylococcus species was conducted using the API – Staph gallery and the enterotoxins secretion using TECRA immunoenzymatic test. Results and discussions The obtained data reveal differences between strains, differences implying the capacity of pathogenic factors synthesis (table 1). If the majority of coagulaso-positive strains elaborated hemolysins (87,88%), only 60,67 % synthesized enterotoxins. It is to be mentioned that only few coagulaso-negative strains synthesized hemolysins (2,5%) and enterotoxins (5,00%). |
