التفاصيل البيبلوغرافية
| العنوان: |
Characterization of chemical exchange between soluble and aggregated states of beta-amyloid by solution-state NMR upon variation of salt conditions |
| المؤلفون: |
Saravanakumar Narayanan, Bernd Reif |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
http://www.chem.uwec.edu/Chem455_S05/Pages/articles/narayanan_%26_reif_2005.pdfTest. |
| سنة النشر: |
2005 |
| المجموعة: |
CiteSeerX |
| الوصف: |
Alzheimer’s disease (AD) is characterized by the accumulation of insoluble fibrillar aggregates of â-amyloid peptides (Aâ), a 39-42 residue peptide, in the brain of AD patients. It is hypothesized that the disease causing form is not the fibrillar species but an oligomeric Aâ molecule, which is often referred to as the “critical oligomer ” of Aâ. We show in this paper that Aâ1-40 undergoes chemical exchange between a monomeric, soluble state and an oligomeric, aggregated state under physiological conditions. In circular dichroism spectroscopy, we observe for this intermediate an R-helical structure. The oligomer is assigned a molecular weight of>100 kDa by diffusion-ordered spectroscopy-solution-state NMR spectroscopy (NMR). We can show by saturation transfer difference NMR experiments that the oligomer is related to monomeric Aâ. This experiment also allows us to identify the chemical groups that are involved in interactions between mono- and oligomeric Aâ molecules. Variation of the anionic strength in the buffer induces a shift of equilibrium between mono- and oligomeric states and possibly allows for the stabilization of these intermediate structures. Alzheimer’s disease (AD)1 is the most abundant age-related neurodegenerative disease. The â-amyloid peptide (Aâ), which exists in different lengths (39-43 amino acids) |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.577.416Test; http://www.chem.uwec.edu/Chem455_S05/Pages/articles/narayanan_%26_reif_2005.pdfTest |
| الإتاحة: |
http://www.chem.uwec.edu/Chem455_S05/Pages/articles/narayanan_%26_reif_2005.pdfTest |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.4BF52518 |
| قاعدة البيانات: |
BASE |
