Noninvasive optical inhibition with a red-shifted microbial rhodopsin ; Nat Neurosci
| العنوان: | Noninvasive optical inhibition with a red-shifted microbial rhodopsin ; Nat Neurosci |
|---|---|
| المساهمون: | Chuong, Amy S, Miri, Mitra L, Busskamp, Volker, Matthews, Gillian A C, Acker, Leah C, Sørensen, Andreas T, Young, Andrew, Klapoetke, Nathan C, Henninger, Mike A, Kodandaramaiah, Suhasa B, Ogawa, Masaaki, Ramanlal, Shreshtha B, Bandler, Rachel C, Allen, Brian D, Forest, Craig R, Chow, Brian Y, Han, Xue, Lin, Yingxi, Tye, Kay M, Roska, Botond, Cardin, Jessica A, Boyden, Edward S |
| المصدر: | Nat Neurosci. 17(8):1123-1129. |
| المجموعة: | CDC Stacks (Centers for Disease Control and Prevention) |
| مصطلحات موضوعية: | Article, Animals, Brain Chemistry, Halobacterium salinarum, Halorhodopsins, Mice, Molecular Sequence Data, Neural Inhibition, Neurons, Optogenetics, Retina |
| الوصف: | Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience. ; 1DP2NS082126/DP/NCCDPHP CDC HHS/United States ; 1DP2OD002002/OD/NIH HHS/United States ; 1R01DA029639/DA/NIDA NIH HHS/United States ; 1R01NS067199/NS/NINDS NIH HHS/United States ; 1R01NS075421/NS/NINDS NIH HHS/United States ; 1R01NS081716/NS/NINDS NIH HHS/United States ; 1R01NS087950/NS/NINDS NIH HHS/United States ; 1R21NS078660/NS/NINDS NIH HHS/United States ; 1RC1MH088182/MH/NIMH NIH HHS/United States ; 5R00MH085944/MH/NIMH NIH HHS/United States ; DP2 DK102256/DK/NIDDK NIH HHS/United States ; DP2 NS082126/NS/NINDS NIH HHS/United States ; DP2-OD-017366-01/OD/NIH HHS/United States ; P30 ES002109/ES/NIEHS NIH HHS/United States ; R00 EY018407/EY/NEI NIH HHS/United States ; R01 EY022951/EY/NEI NIH HHS/United States ; R01 EY022951/EY/NEI NIH HHS/United States ; R01 MH091220-01/MH/NIMH NIH HHS/United States ; R01 MH102365/MH/NIMH NIH HHS/United States ; R01 MH102365/MH/NIMH NIH HHS/United States ; R01 MH102441/MH/NIMH NIH HHS/United States ; R01-MH102441-01/MH/NIMH NIH HHS/United States ; 2014-10-03T00:00:00Z ; 24997763 ; PMC4184214 |
| نوع الوثيقة: | other/unknown material |
| اللغة: | unknown |
| العلاقة: | http://stacks.cdc.gov/view/cdc/30136Test/ |
| الإتاحة: | http://stacks.cdc.gov/view/cdc/30136Test/ |
| رقم الانضمام: | edsbas.6D440C67 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |