رسالة جامعية
Developing a Novel and Facile Method for DNA Isolation from Passive Drool and Oral Swab Samples
| العنوان: | Developing a Novel and Facile Method for DNA Isolation from Passive Drool and Oral Swab Samples |
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| المؤلفون: | Peckels, Caroline |
| المساهمون: | Hodges, Eric |
| بيانات النشر: | University of North Carolina at Chapel Hill |
| سنة النشر: | 2015 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Biobehavioral Research, Salivary Samples, Nursing, DNA Isolation, Epigenetics |
| الوصف: | In 2011, the National Institute of Nursing Research (NINR) held a meeting to develop a five year strategic plan for advancing nursing research in order to help current and future health care needs. At its core, the NINR’s purpose is to improve the health of individuals, families, and communities by funding research projects that focus on new health care initiatives. One novel and noninvasive way of studying genomic contributions to different health conditions is through the use of saliva. In addition to secretions and proteins, saliva also contains essential biomarkers, steroid hormones, antibodies, enzymes, and genetic material that allow researchers to explore deeper into different diseases and conditions. Surprisingly, these salivary sample biomarkers have not been significantly explored until the early 2000s. Due to its minimal invasiveness and ease of use, salivary samples have the potential to be used in vastly different disciplines such as psychology, nursing, medicine, dentistry, and neuroscience. Because of its tremendous potential impact on global diseases and chronic conditions, future research is needed on developing a reproducible method for DNA extraction from whole cell salivary samples. The purpose of this study was twofold: 1) to develop an economical and facile method for extracting DNA from passive drool samples and 2) to develop an economical and facile method for extracting DNA from SalivaBio swabs. By developing these methods, the Biobehavioral research lab will be able to be cost-effective in doing DNA extraction/isolation “in house” and will be able to utilize previous unpreserved samples for future analysis ; Bachelor of Arts |
| نوع الوثيقة: | thesis |
| اللغة: | unknown |
| العلاقة: | https://doi.org/10.17615/699e-ek87Test; https://cdr.lib.unc.edu/downloads/9w032720s?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/9w032720sTest |
| DOI: | 10.17615/699e-ek87 |
| الإتاحة: | https://doi.org/10.17615/699e-ek87Test https://cdr.lib.unc.edu/downloads/9w032720s?file=thumbnailTest https://cdr.lib.unc.edu/downloads/9w032720sTest |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.55249D36 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/699e-ek87 |
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