دورية أكاديمية
CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus
| العنوان: | CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus |
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| المؤلفون: | Graziano V.R, Walker F.C, Kennedy E.A, Wei J, Ettayebi K, Strine M.S, Filler R.B, Hassan E, Hsieh L.L, Kim A.S, Kolawole A.O, Wobus C.E, Lindesmith L.C, Baric R.S, Estes M.K, Orchard R.C, Baldridge M.T, Wilen C.B |
| المصدر: | PLoS Pathogens, 16(4) |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2020 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | bone marrow derived macrophage, human cell, CLM-1 protein, mouse, STAT1 protein, virus RNA, Mice, HeLa cell line, Viral Tropism, virus capsid, Host-Pathogen Interactions, nonhuman, animal model, ascending colon, Host Specificity, Murine norovirus, virology, ileum, Caliciviridae Infections, knockout mouse, controlled study, animal, Receptors, Immunologic, virus pathogenesis, in vitro study, growth, development and aging, human, stomach mucin |
| الوصف: | Murine norovirus (MNoV) is an important model of human norovirus (HNoV) and mucosal virus infection more broadly. Viral receptor utilization is a major determinant of cell tropism, host range, and pathogenesis. The bona fide receptor for HNoV is unknown. Recently, we identified CD300lf as a proteinaceous receptor for MNoV. Interestingly, its paralogue CD300ld was also sufficient for MNoV infection in vitro. Here we explored whether CD300lf is the sole physiologic receptor in vivo and whether HNoV can use a CD300 ortholog as an entry receptor. We report that both CD300ld and CD300lf are sufficient for infection by diverse MNoV strains in vitro. We further demonstrate that CD300lf is essential for both oral and parenteral MNoV infection and to elicit anti-MNoV humoral responses in vivo. In mice deficient in STAT1 signaling, CD300lf is required for MNoV-induced lethality. Finally, we demonstrate that human CD300lf (huCD300lf) is not essential for HNoV infection, nor does huCD300lf inhibit binding of HNoV virus-like particles to glycans. Thus, we report huCD300lf is not a receptor for HNoV. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/8b1q-yx02Test; https://cdr.lib.unc.edu/downloads/d791st20c?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/d791st20cTest |
| DOI: | 10.17615/8b1q-yx02 |
| الإتاحة: | https://doi.org/10.17615/8b1q-yx02Test https://cdr.lib.unc.edu/downloads/d791st20c?file=thumbnailTest https://cdr.lib.unc.edu/downloads/d791st20cTest |
| رقم الانضمام: | edsbas.7AB5C870 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/8b1q-yx02 |
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