المؤلفون: Casiraghi, Monica, Tatreau, Jason R., Abano, John B., Blackwell, John W., Watson, Larry, Burridge, Keith, Randell, Scott H., Egan, Thomas M.

المصدر: The Journal of Thoracic and Cardiovascular Surgery, 138(3)

مصطلحات موضوعية: α, IκBα, ERK, mitogen-activated protein kinase, Cardiology and Cardiovascular Medicine, JNK, bronchiolitis obliterans syndrome, NF, Janus kinase, AM, alveolar macrophage, nuclear factor, HUVEC, lactate dehydrogenase, LDH, ischemia–reperfusion injury, HMVEC, BOS, human pulmonary microvascular endothelial cell, Pulmonary and Respiratory Medicine, lung transplantation, MAPK, human umbilical vein endothelial cell, interleukin, PBS, extracellular signal-regulated kinase, inhibitor of nuclear factor κB, IL, LTX, Surgery

الوصف: Although anoxia/reoxygenation of cultured cells has been employed to model lung ischemia-reperfusion injury (IRI), this does not accurately mimic events experienced by lung cells while a lung is retrieved from a donor, stored, and transplanted. We developed an in vitro model of non-hypoxic IRI to simulate these events.

العلاقة: https://doi.org/10.17615/hc1a-bj24Test; https://cdr.lib.unc.edu/downloads/mw22vf040?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/mw22vf040Test

الإتاحة: https://doi.org/10.17615/hc1a-bj24Test
https://cdr.lib.unc.edu/downloads/mw22vf040?file=thumbnailTest
https://cdr.lib.unc.edu/downloads/mw22vf040Test

المؤلفون: Machado, M., Vigo, J. F.F., Breder, A. C., Simões, J. R., Ximenes, M. C., Hackney, A. C.

المساهمون: University of North Carolina at Chapel Hill

المصدر: Biology of Sport / Institute of Sport, 26(1)

مصطلحات موضوعية: Creatine Kinase, Caffeine, lactate dehydrogenase, Soccer, Transaminases, Exercise

الوصف: Aim: To evaluate the effect of oral caffeine supplementation and strenuous intermittent exercise on muscle damage markers in soccer players. Materials and Methods: 15 male professional soccer players completed a placebo controlled double blind test protocol. At 45 min before exercise, participants ingested 5.5 mg•kg-1 body mass of caffeine (CAF, n=8) or cellulose (CEL, n=7). The exercise was 2 trials of 6 sets of 10 sprints (20 m each) with 10 s recovery time between sprints, 2 min between sets and 15 min between trials. Blood samples were collected before (PRE), 24, 48 and 72 h after exercise. Serum activity of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransaminase (AST), and alanine aminotrasaminase (ALT) were quantified. Results: Serum enzyme activity was enhanced by exercise in both groups, without a synergistic effect of caffeine. Conclusion: Our results suggest muscle damage markers increases after physical activities, but caffeine supplementation (5.5 mg•kg-1 body mass) has no influence upon serum enzymes reflective of muscle integrity and damage.

العلاقة: https://doi.org/10.17615/rff8-sz97Test; https://cdr.lib.unc.edu/downloads/3484zs192?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/3484zs192Test

الإتاحة: https://doi.org/10.17615/rff8-sz97Test
https://cdr.lib.unc.edu/downloads/3484zs192?file=thumbnailTest
https://cdr.lib.unc.edu/downloads/3484zs192Test

المؤلفون: Lay, J.C., Bennett, W.D., Ghio, A.J., Bromberg, P.A., Costa, D.L., Kim, C.S., Koren, H.S., Devlin, R.B.

المصدر: American Journal of Respiratory Cell and Molecular Biology, 20(4)

مصطلحات موضوعية: Inflammation, Macrophages, Alveolar, Time Factors, L-Lactate Dehydrogenase, Interleukin-8, Iron, Lung, Animals, Ferric Compounds, Bronchoalveolar Lavage Fluid, Phagocytosis, Female, Bronchoalveolar Lavage, Instillation, Drug, Adult, Rats, Male, Dinoprostone, Leukotriene C4, Humans, Neutrophils, Leukotriene E4

الوصف: Bronchoalveolar lavage (BAL) was used to sample lung cells and biochemical components in the lung air spaces at various times from 1 to 91 d after intrapulmonary instillation of 2.6 μm-diameter iron oxide particles in human subjects. The instillation of particles induced transient acute inflammation during the first day post instillation (PI), characterized by increased numbers of neutrophils and alveolar macrophages as well as increased amounts of protein, lactate dehydrogenase, and interleukin-8 in BAL fluids. This response was subclinical and was resolved within 4 d PI. A similar dose-dependent response was seen in rats 1 d after intratracheal instillation of the same particles. The particles contained small amounts of soluble iron (240 ng/mg) and possessed the capacity to catalyze oxidant generation in vitro. Our findings indicate that the acute inflammation after particle exposure may, at least partially, be the result of oxidant generation catalyzed by the presence of residual amounts of ferric ion, ferric hydroxides, or oxyhydroxides associated with the particles. These findings may have relevance to the acute health effects associated with increased levels of ambient particulate air pollutants.

العلاقة: https://doi.org/10.17615/rk8a-sb62Test; https://cdr.lib.unc.edu/downloads/9880w072d?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/9880w072dTest

الإتاحة: https://doi.org/10.17615/rk8a-sb62Test
https://cdr.lib.unc.edu/downloads/9880w072d?file=thumbnailTest
https://cdr.lib.unc.edu/downloads/9880w072dTest

المؤلفون: Nelson, Karen G., Siegfried, Jill M., Siegal, Gene P., Becker, Robert, Walton, Leslie A., Kaufman, David G.

المصدر: The American Journal of Pathology, 116(1)

مصطلحات موضوعية: Sarcoma, Lactato deshidrogenasa, Lignée cellulaire, Isozyme, Enzymatic activity, Activité enzymatique, Tumor, Appareil génital femelle pathologie, Human, Isozima, Homme, Utérus, Linea celular, Female genital diseases, Tumeur, Enzyme, Cell line, Heterogeneity, Hétérogénéité, Uterus, Sarcome, Lactate dehydrogenase, Utero

الوصف: Both the tissue lactate dehydrogenase (LDH) isoenzyme pattern and total LDH activity were investigated in a series of human uterine sarcomas and cell lines derived from these tumors. Abnormal LDH isoenzyme patterns characterized by shifts toward the muscle isoenzyme forms (LDH4 and 5) were found in some, but not all, of the sarcomas. The tumors containing rhabdomyosarcomatous elements usually had normal or only slightly altered LDH isoenzyme patterns. The LDH isoenzyme patterns expressed by cultured cell lines established from the uterine sarcomas, in general, resembled those found in the primary tumors. These results suggest that the normal or near normal LDH isoenzyme distributions found in uterine sarcomas with rhabdomyosarcomatous differentiation may be a potential characteristic which may be used for distinguishing these tumors from other uterine sarcomas.

العلاقة: https://doi.org/10.17615/za1f-st03Test; https://cdr.lib.unc.edu/downloads/kw52jh20h?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/kw52jh20hTest

الإتاحة: https://doi.org/10.17615/za1f-st03Test
https://cdr.lib.unc.edu/downloads/kw52jh20h?file=thumbnailTest
https://cdr.lib.unc.edu/downloads/kw52jh20hTest

المؤلفون: Fu, Kai-Song, Hassett, Daniel J., Cohen, Myron S.

المصدر: Infection and Immunity, 57(7)

مصطلحات موضوعية: Hiperóxido, Neisseria gonorrhoeae, Résistance, Hydrogen Peroxides, Enzyme, Neisseriaceae, Enzymatic activity, Adaptation, Bacteria, Hyperoxyde, Resistance, Hyperoxides, Milieu oxydant, Métabolisme, Bactérie, Oxidizing environment, Lactate dehydrogenase, Medio oxidante, Hydrogène Peroxyde, Metabolism, Activité enzymatique, Micrococcales

الوصف: Neisseria gonorrhoeae, an obligate human pathogen, is subjected to oxidant stress when attacked by 02 reduction products formed by neutrophils. In this study, exposure of gonococci to sublethal concentrations of superoxide and hydrogen peroxide (and related 0-centered radicals) resulted in phenotypic resistance to oxidant stress. Adaptation required new protein formation but was not related to increases in superoxide dismutase or catalase. We have previously demonstrated that gonococci use phagocyte-derived L-(+)-lactate. Oxidant stress of greater magnitude than that required for adaptation led to a generalized increase in bacterial metabolism, particularly in L-(+)- and D-(-)-lactate utilization and lactate dehydrogenase activity. Increased lactate utilization required new protein synthesis. These results suggest the possibility that lactate metabolism is of importance to N. gonorrhoeae subjected to oxidant stress. Use of Ict mutant organisms unable to use L-(+)-lactate should allow examination of this hypothesis.

العلاقة: https://doi.org/10.17615/yedf-7j94Test; https://cdr.lib.unc.edu/downloads/cf95jj972?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/cf95jj972Test

الإتاحة: https://doi.org/10.17615/yedf-7j94Test
https://cdr.lib.unc.edu/downloads/cf95jj972?file=thumbnailTest
https://cdr.lib.unc.edu/downloads/cf95jj972Test