دورية أكاديمية
Directing the biological activities of heparan sulfate oligosaccharides using a chemoenzymatic approach
| العنوان: | Directing the biological activities of heparan sulfate oligosaccharides using a chemoenzymatic approach |
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| المؤلفون: | Xu, Yongmei, Wang, Zhen, Liu, Renpeng, Bridges, Arlene S, Huang, Xuefei, Liu, Jian |
| المصدر: | Glycobiology, 22(1) |
| سنة النشر: | 2012 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Protein Binding, Biocatalysis, Carbohydrate Conformation, Sulfuric Acid Esters, Molecular Sequence Data, Racemases and Epimerases, Antithrombins, Glycosyltransferases, Heparitin Sulfate, Fibroblast Growth Factor 2, Sulfotransferases, Carbohydrate Sequence |
| الوصف: | Heparan sulfate (HS) and heparin are highly sulfated polysaccharides exhibiting essential physiological functions. The sulfation patterns determine the functional selectivity for HS and heparin. Chemical synthesis of HS, especially those larger than a hexasaccharide, remains challenging. Enzymatic synthesis of HS has recently gained momentum. Here we describe the divergent assembly of HS heptasaccharides and nonasaccharides from a common hexasaccharide precursor. The hexasaccharide precursor was synthesized via a chemical method. The subsequent elongation, sulfation and epimerization were completed by glycosyltransferases, HS sulfotransferases and epimerase. Using the synthesized heptasaccharides, we discovered that the iduronic acid is critical for binding to fibroblast growth factor-2. We also designed a synthetic path to prepare a nonasaccharide with an antithrombin-binding affinity of 3 nM. Our method demonstrated the feasibility of combining chemical and enzymatic synthesis to prepare structurally defined HS oligosaccharides with desired biological activities. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/9pbt-zw62Test; https://cdr.lib.unc.edu/downloads/xg94hw655?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/xg94hw655Test |
| DOI: | 10.17615/9pbt-zw62 |
| الإتاحة: | https://doi.org/10.17615/9pbt-zw62Test https://cdr.lib.unc.edu/downloads/xg94hw655?file=thumbnailTest https://cdr.lib.unc.edu/downloads/xg94hw655Test |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.DD8E982C |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/9pbt-zw62 |
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