دورية أكاديمية
Akt-dependent Activation of mTORC1 Complex Involves Phosphorylation of mTOR (Mammalian Target of Rapamycin) by IκB Kinase α (IKKα)
| العنوان: | Akt-dependent Activation of mTORC1 Complex Involves Phosphorylation of mTOR (Mammalian Target of Rapamycin) by IκB Kinase α (IKKα) |
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| المؤلفون: | Dan, Han C., Ebbs, Aaron, Pasparakis, Manolis, Van Dyke, Terry, Basseres, Daniela S., Baldwin, Albert S. |
| المصدر: | The Journal of Biological Chemistry, 289(36) |
| سنة النشر: | 2014 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | HEK293 Cells, IKK, Akt, Cell Line, Tumor, Cells, Cultured, Mammalian Target of Rapamycin (mTOR), Mice, Knockout, Adaptor Proteins, Signal Transducing, Humans, Female, Male, Animals, Raptor, I-kappa B Kinase, Phosphatase and Tensin Homolog (PTEN), Cell Proliferation, Mechanistic Target of Rapamycin Complex 1, Immunoblotting, HeLa Cells, Enzyme Activation, Phosphorylation |
| الوصف: | The serine/threonine protein kinase Akt promotes cell survival, growth, and proliferation through phosphorylation of different downstream substrates. A key effector of Akt is the mammalian target of rapamycin (mTOR). Akt is known to stimulate mTORC1 activity through phosphorylation of tuberous sclerosis complex 2 (TSC2) and PRAS40, both negative regulators of mTOR activity. We previously reported that IκB kinase α (IKKα), a component of the kinase complex that leads to NF-κB activation, plays an important role in promoting mTORC1 activity downstream of activated Akt. Here, we demonstrate IKKα-dependent regulation of mTORC1 using multiple PTEN null cancer cell lines and an animal model with deletion of IKKα. Importantly, IKKα is shown to phosphorylate mTOR at serine 1415 in a manner dependent on Akt to promote mTORC1 activity. These results demonstrate that IKKα is an effector of Akt in promoting mTORC1 activity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/3jnj-sd33Test; https://cdr.lib.unc.edu/downloads/gq67k031f?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/gq67k031fTest |
| DOI: | 10.17615/3jnj-sd33 |
| الإتاحة: | https://doi.org/10.17615/3jnj-sd33Test https://cdr.lib.unc.edu/downloads/gq67k031f?file=thumbnailTest https://cdr.lib.unc.edu/downloads/gq67k031fTest |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.15680648 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/3jnj-sd33 |
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