دورية أكاديمية
Solution Structures of Chemoenzymatically Synthesized Heparin and Its Precursors
| العنوان: | Solution Structures of Chemoenzymatically Synthesized Heparin and Its Precursors |
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| المؤلفون: | Zhang, Zhenqing, McCallum, Scott A., Xie, Jin, Nieto, Lidia, Corzana, Francisco, Jiménez-Barbero, Jesús, Chen, Miao, Liu, Jian, Linhardt, Robert J. |
| المصدر: | Journal of the American Chemical Society, 130(39) |
| سنة النشر: | 2008 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Oligosaccharides, Heparin, Animals, Carbohydrate Conformation, Models, Molecular, Mice, Heparitin Sulfate, Carbon Isotopes, Glycosaminoglycans, Sulfotransferases, Nitrogen Isotopes, Carbohydrate Epimerases, Humans, Solutions, Nuclear Magnetic Resonance, Biomolecular |
| الوصف: | We report the first chemoenzymatic synthesis of the stable isotope-enriched heparin from a uniformly labeled [13C,15N]N-acetylheparosan (-GlcA(1,4)GlcNAc-) prepared from E. coli K5. Glycosaminoglycan (GAG) precursors and heparin were formed from N-acetylheparosan by the following steps: chemical N-deacetylation and N-sulfonation leading to N-sulfoheparosan (-GlcA(1,4)GlcNS-); enzyme-catalyzed C5-epimerization and 2-O-sulfonation leading to undersulfated heparin (-IdoA2S(1,4)GlcNS-); enzymatic 6-O-sulfonation leading to the heparin backbone (-IdoA2S(1,4)GlcNS6S-); and selective enzymatic 3-O-sulfonation leading to the anticoagulant heparin, containing the GlcNS6S3S residue. Heteronuclear, multidimensional nuclear magnetic resonance spectroscopy was employed to analyze the chemical composition and solution structure of [13C,15N]N-acetylheparosan, precursors, and heparin. Isotopic enrichment was found to provide well-resolved 13C spectra with the high sensitivity required for conformational studies of these biomolecules. Stable isotope-labeled heparin was indistinguishable from heparin derived from animal tissues and is a novel reagent for studying the interaction of heparin with proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/dfhs-5271Test; https://cdr.lib.unc.edu/downloads/fb494h16g?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/fb494h16gTest |
| DOI: | 10.17615/dfhs-5271 |
| الإتاحة: | https://doi.org/10.17615/dfhs-5271Test https://cdr.lib.unc.edu/downloads/fb494h16g?file=thumbnailTest https://cdr.lib.unc.edu/downloads/fb494h16gTest |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.A2A9502E |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/dfhs-5271 |
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