دورية أكاديمية
CcpA-Independent Glucose Regulation of Lactate Dehydrogenase 1 in Staphylococcus aureus
| العنوان: | CcpA-Independent Glucose Regulation of Lactate Dehydrogenase 1 in Staphylococcus aureus |
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| المؤلفون: | Crooke, Adrianne K., Fuller, James R., Obrist, Markus W., Tomkovich, Sarah E., Vitko, Nicholas P., Richardson, Anthony R. |
| المصدر: | PloS One, 8(1) |
| سنة النشر: | 2013 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Gene Expression Regulation, Bacterial, lactate dehydrogenase 1, Glucose, Staphylococcus aureus, Isoenzymes, L-Lactate Dehydrogenase, Bacterial Proteins |
| الوصف: | Lactate Dehydrogenase 1 (Ldh1) is a key enzyme involved in Staphylococcus aureus NO·-resistance. Full ldh1-induction requires the presence of glucose, and mutants lacking the Carbon-Catabolite Protein (CcpA) exhibit decreased ldh1 transcription and diminished Ldh1 activity. The redox-regulator Rex represses ldh1 directly by binding to Rex-sites within the ldh1 promoter (Pldh1). In the absence of Rex, neither glucose nor CcpA affect ldh1 expression implying that glucose/CcpA-mediated activation requires Rex activity. Rex-mediated repression of ldh1 depends on cellular redox status and is maximal when NADH levels are low. However, compared to WT cells, the ΔccpA mutant exhibited impaired redox balance with relatively high NADH levels, yet ldh1 was still poorly expressed. Furthermore, CcpA did not drastically alter Rex transcript levels, nor did glucose or CcpA affect the expression of other Rex-regulated genes indicating that the glucose/CcpA effect is specific for Pldh1. A putative catabolite response element (CRE) is located ∼30 bp upstream of the promoter-distal Rex-binding site in Pldh1. However, CcpA had no affinity for Pldh1 in vitro and a genomic mutation of CRE upstream of Pldh1 in S. aureus had no affect on Ldh1 expression in vivo. In contrast to WT, ΔccpA S. aureus preferentially consumes non-glycolytic carbon sources. However when grown in defined medium with glucose as the primary carbon source, ΔccpA mutants express high levels of Ldh1 compared to growth in media devoid of glucose. Thus, the actual consumption of glucose stimulates Ldh1 expression rather than direct CcpA interaction at Pldh1. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/yf1c-x766Test; https://cdr.lib.unc.edu/downloads/794085230?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/794085230Test |
| DOI: | 10.17615/yf1c-x766 |
| الإتاحة: | https://doi.org/10.17615/yf1c-x766Test https://cdr.lib.unc.edu/downloads/794085230?file=thumbnailTest https://cdr.lib.unc.edu/downloads/794085230Test |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.88F82094 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/yf1c-x766 |
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