دورية أكاديمية
Enhancer viruses for combinatorial cell-subclass-specific labeling
| العنوان: | Enhancer viruses for combinatorial cell-subclass-specific labeling |
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| المؤلفون: | Graybuck, Lucas T., Daigle, Tanya L., Sedeño-Cortés, Adriana E., Walker, Miranda, Kalmbach, Brian, Lenz, Garreck H., Morin, Elyse, Nguyen, Thuc Nghi, Garren, Emma, Bendrick, Jacqueline L., Kim, Tae Kyung, Zhou, Thomas, Mortrud, Marty, Yao, Shenqin, Sieverts, La'Akea, Larsen, Rachael, Gore, Bryan B., Szelenyi, Eric R., Trader, Cameron, Balaram, Pooja, van Velthoven, Cindy T. J., Chiang, Megan, Mich, John K., Dee, Nick, Goldy, Jeff, Cetin, Ali H., Smith, Kimberly, Way, Sharon W., Esposito, Luke, Yao, Zizhen, Gradinaru, Viviana, Sunkin, Susan M., Lein, Ed, Levi, Boaz P., Ting, Jonathan T., Zeng, Hongkui, Tasic, Bosiljka |
| المصدر: | Neuron, 109(9), 1449-1464, (2021-05-05) |
| بيانات النشر: | Cell Press |
| سنة النشر: | 2021 |
| المجموعة: | Caltech Authors (California Institute of Technology) |
| مصطلحات موضوعية: | AAV, enhancer, transgenic mouse, cell types, recombinase, ATAC-seq, cortex |
| الوصف: | Rapid cell type identification by new genomic single-cell analysis methods has not been met with efficient experimental access to these cell types. To facilitate access to specific neural populations in mouse cortex, we collected chromatin accessibility data from individual cells and identified enhancers specific for cell subclasses and types. When cloned into recombinant adeno-associated viruses (AAVs) and delivered to the brain, these enhancers drive transgene expression in specific cortical cell subclasses. We extensively characterized several enhancer AAVs to show that they label different projection neuron subclasses as well as a homologous neuron subclass in human cortical slices. We also show how coupling enhancer viruses expressing recombinases to a newly generated transgenic mouse, Ai213, enables strong labeling of three different neuronal classes/subclasses in the brain of a single transgenic animal. This approach combines unprecedented flexibility with specificity for investigation of cell types in the mouse brain and beyond. ; © 2021 Elsevier Inc. Received 17 April 2020, Revised 14 December 2020, Accepted 8 March 2021, Available online 30 March 2021. We could not have performed this study without the support of the following Allen Institute teams and departments: Lab Animal Services, Transgenic Colony Management, Tissue Processing, FACS Core, Molecular Biology, Molecular Genetics, and Human Cell Types. We thank Aaron Oster for Addgene reagent submission; Dr. Andrew Ko and Dr. C. Dirk Keene and associated teams at Harborview Medical Center (UW Medicine) for providing the human surgical tissue specimen in this study; Andrew Hill and Darren Cusanovich for assistance with data from Cusanovich et al. (2018), and Advanced Cell Diagnostics for early access to RNAscope HiPlex. The project described was supported by award number R01DA036909 from the National Institute on Drug Abuse to B.T. and H.Z.; by National Institutes of Health (NIH) BRAIN Initiative award RF1MH121274 to B.T., T.L.D., and H.Z.; and by ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://doi.org/10.1101/525014Test; https://doi.org/10.1016/j.neuron.2021.03.011Test; oai:authors.library.caltech.edu:k5yvf-a4q19; https://www.ncbi.nlm.nih.gov/pmc/PMC8610077Test; eprintid:102693; resolverid:CaltechAUTHORS:20200421-092814882 |
| DOI: | 10.1016/j.neuron.2021.03.011 |
| الإتاحة: | https://doi.org/10.1016/j.neuron.2021.03.01110.1101/525014Test https://www.ncbi.nlm.nih.gov/pmc/PMC8610077Test |
| حقوق: | info:eu-repo/semantics/openAccess ; Other |
| رقم الانضمام: | edsbas.460930E6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.neuron.2021.03.011 |
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