دورية أكاديمية
The predominance of Ethiopian specific Mycobacterium tuberculosis families and minimal contribution of Mycobacterium bovis in tuberculous lymphadenitis patients in Southwest Ethiopia
| العنوان: | The predominance of Ethiopian specific Mycobacterium tuberculosis families and minimal contribution of Mycobacterium bovis in tuberculous lymphadenitis patients in Southwest Ethiopia |
|---|---|
| المؤلفون: | Tadesse, M., Abebe, G., Bekele, A., Bezabih, M., de Rijk, P., Meehan, Conor J., de Jong, B.C., Rigouts, L. |
| سنة النشر: | 2019 |
| المجموعة: | Bradford Scholars@University of Bradford |
| مصطلحات موضوعية: | Mycobacterium tuberculosis, Mycobacterium bovis, Tuberculous lymphadenitis, Ethiopia |
| الوصف: | No ; Background: Ethiopia has an extremely high rate of extrapulmonary tuberculosis, dominated by tuberculous lymphadenitis (TBLN). However, little is known about Mycobacterium tuberculosis complex (MTBc) lineages re-sponsible for TBLN in Southwest Ethiopia.Methods:A total of 304 MTBc isolates from TBLN patients in Southwest Ethiopia were genotyped primarily by spoligotyping. Isolates of selected spoligotypes were further analyzed by 15-loci mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (n = 167) and qPCR-based single nucleotide polymorphism (n = 38). Isolates were classified into main phylogenetic lineages and families by using the re-ference strain collections and identification tools available at MIRU-VNTRplus data base. Resistance to rifampicin was determined by Xpert MTB/RIF. Results: The majority of isolates (248; 81.6%) belonged to the Euro-American lineage (Lineage 4), with the ill-defined T and Haarlem as largest families comprising 116 (38.2%) and 43 (14.1%) isolates respectively. Of the T family, 108 isolates were classified as being part of the newly described Ethiopian families, namely Ethiopia_2(n = 44), Ethiopia_3 (n = 34) and Ethiopia_H37Rv-like (n = 30). Other sub-lineages included URAL (n = 18), S(n = 17), Uganda I (n = 16), LAM (n = 13), X (n = 5), TUR (n = 5), Uganda II (n = 4) and unknown (n = 19).Lineage 3 (Delhi/CAS) was the second most common lineage comprising 44 (14.5%) isolates. Interestingly, six isolates (2%) were belonged to Lineage 7, unique to Ethiopia. Lineage 1 (East-African Indian) and Lineage 2(Beijing) were represented by 3 and 1 isolates respectively.M. bovis was identified in only two (0.7%) TBLN cases. The cluster rate was highest for Ethiopia_3 isolates showing clonal similarity with isolates from North Ethiopia. Lineage 3 was significantly associated with rifampicin resistance. Conclusions: In TBLN in Southwest Ethiopia, the recently described Ethiopia specific Lineage 4 families were predominant, followed by Lineage 3 and Lineage ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Tadesse M, Abebe G, Bekele A et al (2017) The predominance of Ethiopian specific Mycobacterium tuberculosis families and minimal contribution of Mycobacterium bovis in tuberculous lymphadenitis patients in Southwest Ethiopia. Infection, Genetics and Evolution. 55: 251-259.; http://hdl.handle.net/10454/17305Test; https://doi.org/10.1016/j.meegid.2017.09.016Test |
| DOI: | 10.1016/j.meegid.2017.09.016 |
| الإتاحة: | https://doi.org/10.1016/j.meegid.2017.09.016Test http://hdl.handle.net/10454/17305Test |
| رقم الانضمام: | edsbas.8E3EEF0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.meegid.2017.09.016 |
|---|