التفاصيل البيبلوغرافية
| العنوان: |
Age and sex differences in kidney microRNA expression during the life span of F344 rats |
| المؤلفون: |
Kwekel, Joshua C, Vijay, Vikrant, Desai, Varsha G, Moland, Carrie L, Fuscoe, James C |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2015 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
Kidney, miRNA expression, Sex, Age, Renal fibrosis, Renal inflammation, Biomarker |
| الوصف: |
Background Growing evidence suggests that epigenetic mechanisms of gene regulation may play a role in susceptibilities to specific toxicities and adverse drug reactions. MiRNAs in particular have been shown to be important regulators in cancer and other diseases and show promise as predictive biomarkers for diagnosis and prognosis. In this study, we characterized the global kidney miRNA expression profile in untreated male and female F344 rats throughout the life span. These findings were correlated with sex-specific susceptibilities to adverse renal events, such as male-biased renal fibrosis and inflammation in old age. Methods Kidney miRNA expression was examined in F344 rats at 2, 5, 6, 8, 15, 21, 78, and 104 weeks of age in both sexes using Agilent miRNA microarrays. Differential expression was determined using filtering criteria of ≥1.5 fold change and ANOVA or pairwise t -test (FDR <5%) to determine significant age and sex effects, respectively. Pathway analysis software was used to investigate the possible roles of these target genes in age- and sex-specific differences. Results Three hundred eleven miRNAs were found to be expressed in at least one age and sex. Filtering criteria revealed 174 differentially expressed miRNAs in the kidney; 173 and 34 miRNAs exhibiting age and sex effects, respectively. Principal component analysis revealed age effects predominated over sex effects, with 2-week miRNA expression being much different from other ages. No significant sexually dimorphic miRNA expression was observed from 5 to 8 weeks, while the most differential expression (13 miRNAs) was observed at 21 weeks. Potential target genes of these differentially expressed miRNAs were identified. Conclusions The expression of 56% of detected renal miRNAs was found to vary significantly with age and/or sex during the life span of F344 rats. Pathway analysis suggested that 2-week-expressed miRNAs may be related to organ and cellular development and proliferation pathways. Male-biased miRNA expression at older ... |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.bsd-journal.com/content/6/1/1Test |
| الإتاحة: |
http://www.bsd-journal.com/content/6/1/1Test |
| حقوق: |
Copyright 2015 Kwekel et al.; licensee Biomed Central. |
| رقم الانضمام: |
edsbas.D111DAB8 |
| قاعدة البيانات: |
BASE |
