التفاصيل البيبلوغرافية
| العنوان: |
Periostin: a promising target of therapeutical intervention for prostate cancer |
| المؤلفون: |
Sun, Chuanyu, Zhao, Xiaojun, Xu, Ke, Gong, Jian, Liu, Weiwei, Ding, Weihong, Gou, Yuancheng, Xia, Guowei, Ding, Qiang |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2011 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
Periostin, Prostate cancer, RNAi, Proliferation, Migration |
| الوصف: |
Background In our recent study, Periostin was up-regulated in prostate cancer(PCa) compared with benign prostate hyperplasia (BPH) by proteomics analysis of prostate biopsies. We investigated the effect of sliencing Periostin by RNA interference (RNAi) on the proliferation and migration of PCa LNCap cell line. Methods All the prostate biopsies from PCa, BPH and BPH with local prostatic intraepithelial neoplasm(PIN) were analyzed by iTRAQ(Isobaric tags for relative and absolute quantification) technology. Western blotting and immunohistochemical staining were used to verify Periostin expression in the tissues of PCa. Periostin expression in different PCa cell lines was determined by immunofluorescence staining, western blotting and reverse transcription PCR(RT-PCR). The LNCap cells with Periostin expression were used for transfecting shRNA-Periostin lentiviral particles. The efficancy of transfecting shRNA lentiviral particles was evaluated by immunofluorescence, western blotting and Real-time PCR. The effect of silencing Periostin expression by RNAi on proliferation of LNCap cells was determined by MTT assay and tumor xenografts. The tissue slices from theses xenografts were analyzed by hematoxylin and eosin(HE) staining. The expression of Periostin in the xenografts was deteminned by Immunohistochemical staining and western blotting. The migration of LNCap cells after silencing Periostin gene expression were analyzed in vitro. Results Periostin as the protein of interest was shown 9.12 fold up-regulation in PCa compared with BPH. The overexpression of Periostin in the stroma of PCa was confirmed by western blotting and immunohistochemical staining. Periostin was only expressed in PCa LNCap cell line. Our results indicated that the transfection ratio was more than 90%. As was expected, both the protein level and mRNA level of Periostin in the stably expressing shRNA-Periostin LNCap cells were significantly reduced. The stably expressing shRNA-Periostin LNCap cells growed slowly in vitro and in vivo. The ... |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.translational-medicine.com/content/9/1/99Test |
| الإتاحة: |
http://www.translational-medicine.com/content/9/1/99Test |
| حقوق: |
Copyright 2011 Sun et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.B152C24F |
| قاعدة البيانات: |
BASE |
