التفاصيل البيبلوغرافية
| العنوان: |
Significant impact of different oxygen breathing conditions on noninvasive in vivotumor-hypoxia imaging using [18F]-fluoro-azomycinarabino-furanoside ([18F]FAZA) |
| المؤلفون: |
Maier, Florian C, Kneilling, Manfred, Reischl, Gerald, Cay, Funda, Bukala, Daniel, Schmid, Andreas, Judenhofer, Martin S, Röcken, Martin, Machulla, Hans-Jürgen, Pichler, Bernd J |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2011 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background [ 18 F]FAZA is a PET biomarker with great potential for imaging tumor hypoxia. Aim of our study was to compare [ 18 F]FAZA uptake in mice with subcutaneous exogenous CT26 colon carcinomas and endogenous polyoma middle-T (PyV-mT) mammary carcinomas and to analyze the influence of different breathing protocols in CT26 colon carcinomas as well as the reversibility or irreversibility of [ 18 F]FAZA uptake. Methods We injected subcutaneous CT26 colon carcinoma or polyomavirus middle-T (PyV-mT) mammary carcinoma-bearing mice intravenously with 18 F-FAZA and performed PET scans 1-3 h post injection ( p.i. ). To analyze the impact of oxygen supply in CT26 carcinomas we used three different breathing protocols: (P0) air; (P1) 100% oxygen 1 h prior injection until 3 h p.i. ; (P2) 100% oxygen breathing starting 2 min prior tracer injection until 1 h p.i. and during the PET scans; mice were breathing air between the 2 h and 3 h 10 min static scans. Normalized PET images were analyzed by using defined regions of interest. Finally, some mice were dissected for pimonidazole immunohistochemistry. Results There was no difference in 18 F-FAZA uptake 1-3 h p.i. between the two carcinoma types (CT26: 1.58 ± 0.45%ID/cc; PyV-mT: 1.47 ± 0.89%ID/cc, 1 h p.i. , tumor size < 0.5 cm 3 ). We measured a significant tracer clearance, which was more pronounced in muscle tissue (P0). The [ 18 F]FAZA tumor-to-muscle-ratios in CT26 colon carcinoma-bearing mice 2 h and 3 h, but not 1 h p.i. were significantly higher when the mice breathed air (P0: 3.56 ± 0.55, 3 h) compared to the oxygen breathing protocols (P1: 2.45 ± 0.58; P2: 2.77 ± 0.42, 3 h). Surprisingly, the breathing protocols P1 and P2 showed no significant differences in T/M ratios, thus indicating that the crucial [ 18 F]FAZA uptake phase is during the first hour after [ 18 F]FAZA injection. Importantly, the muscle clearance was not affected by the different oxygen breathing conditions while the tumor clearance was lower when mice were breathing air. Conclusion ... |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.ro-journal.com/content/6/1/165Test |
| الإتاحة: |
http://www.ro-journal.com/content/6/1/165Test |
| حقوق: |
Copyright 2011 Maier et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.4069AB6F |
| قاعدة البيانات: |
BASE |
