التفاصيل البيبلوغرافية
| العنوان: |
Transforming growth factor beta-1 decreases the yield of the second meiotic division of rat pachytene spermatocytes in vitro |
| المؤلفون: |
Damestoy, Anne, Perrard, Marie-Hélène, Vigier, Michèle, Sabido, Odile, Durand, Philippe |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2005 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background TGF beta and its receptors are present in both germ cells and somatic cells of the male gonad. However, knock-out strategies for studying spermatogenesis regulation by TGF beta have been disappointing since TGF beta-or TGF beta receptor-null mice do not survive longer than a few weeks. Methods In the present study, we addressed the role of TGF beta-1 on the completion of meiosis by rat pachytene spermatocytes (PS) cocultured with Sertoli cells. Identification and counting of meiotic cells were performed by cytology and cytometry. Results Under our culture conditions, some PS differentiated into round spermatids (RS). When TGF beta-1 was added to the culture medium, neither the number of PS or of secondary spermatocytes nor the half-life of RS was modified by the factor. By contrast, the number of RS and the amount of TP1 mRNA were lower in TGF beta-1-treated cultures than in control cultures. Very few metaphase I cells were ever observed both in control and TGF beta-1-treated wells. Higher numbers of metaphase II were present and their number was enhanced by TGF beta-1 treatment. A TGF beta-like bioactivity was detected in control culture media, the concentration of which increased with the time of culture. Conclusion These results indicate that TGF beta-1 did not change greatly, if any, the yield of the first meiotic division but likely enhanced a bottleneck at the level of metaphase II. Taken together, our results suggest strongly that TGF beta participates in an auto/paracrine pathway of regulation of the meiotic differentiation of rat spermatocytes. |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.rbej.com/content/3/1/22Test |
| الإتاحة: |
http://www.rbej.com/content/3/1/22Test |
| حقوق: |
Copyright 2005 Damestoy et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.4F2DDDEA |
| قاعدة البيانات: |
BASE |
