التفاصيل البيبلوغرافية
| العنوان: |
Transcriptional analysis of porcine circovirus-like virus P1 |
| المؤلفون: |
Wen, Li-bin, Wang, Feng-zhi, He, Kong-wang, Li, Bin, Wang, Xiao-min, Guo, Rong-li, Xie, Jian-ping |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2014 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
Porcine circovirus like virus P1, Transcriptional analysis, Northern blotting, RACE |
| الوصف: |
Background Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts. Results The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (−) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids). Conclusions We show a very complex viral transcription pattern in P1-infected cells. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
http://www.biomedcentral.com/1746-6148/10/287Test |
| الإتاحة: |
http://www.biomedcentral.com/1746-6148/10/287Test |
| حقوق: |
Copyright 2014 Wen et al.; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.9D8F8263 |
| قاعدة البيانات: |
BASE |
