التفاصيل البيبلوغرافية
| العنوان: |
Titanium dioxide nanoparticles induce the expression of early and late receptors for adhesion molecules on monocytes |
| المؤلفون: |
Rueda-Romero, Cristhiam, Hernández-Pérez, Guillermina, Ramos-Godínez, Pilar, Vázquez-López, Inés, Quintana-Belmares, Raúl, Huerta-García, Elizabeth, Stepien, Ewa, López-Marure, Rebeca, Montiel-Dávalos, Angélica, Alfaro-Moreno, Ernesto |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2016 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
Titanium dioxide nanoparticles, Monocyte, Endothelial cell, Adhesion molecules, sLex, PSGL-1, LFA-1, VLA-4, αVβ3 |
| الوصف: |
Background There is growing evidence that exposure to titanium dioxide nanoparticles (TiO 2 NPs) could be harmful. Previously, we have shown that TiO 2 NPs induces endothelial cell dysfunction and damage in glial cells. Considering that inhaled particles can induce systemic effects and the evidence that nanoparticles may translocate out of the lungs, we evaluated whether different types of TiO 2 NPs can induce the expression of receptors for adhesion molecules on monocytes (U937 cell line). We evaluated the role of reactive oxygen spices (ROS) on these effects. Methods The expression of receptors for early (sLe x and PSGL-1) and late (LFA-1, VLA-4 and αVβ3) adhesion molecules was evaluated in U937 cells on a time course (3–24 h) using a wide range of concentrations (0.001-100 μg/mL) of three types of TiO 2 NPs (<25 nm anatase, 50 nm anatase-rutile or < 100 nm anatase). Cells exposed to TNFα were considered positive controls, and unexposed cells, negative controls. In some experiments we added 10 μmolar of N-acetylcysteine (NAC) to evaluate the role of ROS. Results All tested particles, starting at a concentration of 0.03 μg/mL, induced the expression of receptors for early and late adhesion molecules. The largest increases were induced by the different molecules after 3 h of exposure for sLe x and PSGL-1 (up to 3-fold of the positive controls) and after 18 h of exposure for LFA-1, VLA-4 and αVβ3 (up to 2.5-fold of the positive controls). Oxidative stress was observed as early as 10 min after exposure, but the maximum peak was found after 4 h of exposure. Adhesion of exposed or unexposed monocytes to unexposed or exposed endothelial cells was tested, and we observed that monocytes cells adhere in similar amounts to endothelial cells if one of the two cell types, or both were exposed. When NAC was added, the expression of the receptors was inhibited. . |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.particleandfibretoxicology.com/content/13/1/36Test |
| الإتاحة: |
http://www.particleandfibretoxicology.com/content/13/1/36Test |
| حقوق: |
Copyright 2016 The Author(s). |
| رقم الانضمام: |
edsbas.BC789980 |
| قاعدة البيانات: |
BASE |
