التفاصيل البيبلوغرافية
| العنوان: |
4′-O-β-d-glucosyl-5-O-methylvisamminol, an active ingredient of Saposhnikovia divaricata, attenuates high-mobility group box 1 and subarachnoid hemorrhage-induced vasospasm in a rat model |
| المؤلفون: |
Chang, Chih-Zen, Wu, Shu-Chuan, Kwan, Aij-Lie, Lin, Chih-Lung |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2015 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
4′-O-β-d-glucosyl-5-O-methylvisamminol, High-mobility group box 1, Tumor necrotic factor-α, Subarachnoid hemorrhage, Vasospasm |
| الوصف: |
Background High-mobility group box 1 (HMGB1) was observed to be an important extracellular mediator involved in vascular inflammation associated with subarachnoid hemorrhage (SAH). This study is of interest to examine the efficacy of 4′-O-β- d -glucosyl-5-O-methylvisamminol (4OGOMV), C 22 H 28 O 10 , on the alternation of cytokines and HMGB1 in an animal model. Methods A rodent double hemorrhage SAH model was employed. Administration with 4OGOMV was initiated 1 h after animals were subjected to SAH. Basilar arteries (BAs) were harvested and cortexes examined for HMGB1 mRNA, protein expression (Western blot) and monocyte chemoattractant protein-1 (MCP-1) immunostaining. Cerebrospinal fluid samples were collected to examine IL-1β, IL-6, IL-8 and MCP-1 (rt-PCR). Results Morphological findings revealed endothelial cell deformity, intravascular elastic lamina torture, and smooth muscle necrosis in the vessels of SAH groups. Correspondently, IL-1β, IL-6 and MCP-1 in the SAH-only and SAH-plus vehicle groups was also elevated. 4OGOMV dose-dependently reduced HMGB1 protein expression when compared with the SAH groups.(p < 0.01) Likewise, 400 μg/kg 4OGOMV reduced IL-1β, MCP-1 and HMGB1 mRNA levels as well as MCP-1(+) monocytes when compared with the SAH groups. Conclusion 4OGOMV exerts its neuro-protective effect partly through the dual effect of inhibiting IL-6 and MCP-1 activation and also reduced HMGB1 protein, mRNA and MCP-1(+) leukocytes translocation. This study lends credence to validating 4OGOMV as able to attenuate pro-inflammatory cytokine mRNA, late-onset inflammasome, and cellular basis in SAH-induced vasospasm. |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.behavioralandbrainfunctions.com/content/11/1/28Test |
| الإتاحة: |
http://www.behavioralandbrainfunctions.com/content/11/1/28Test |
| حقوق: |
Copyright 2015 Chang et al. |
| رقم الانضمام: |
edsbas.BF174E08 |
| قاعدة البيانات: |
BASE |
