التفاصيل البيبلوغرافية
| العنوان: |
Functional analysis of hepatitis B virus pre-s deletion variants associated with hepatocellular carcinoma |
| المؤلفون: |
Lin, Chih-Ming, Wang, Gen-Ming, Jow, Guey-Mei, Chen, Bing-Fang |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2012 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
HBV, hepatocellular carcinoma, pre-S deletion, S promoter |
| الوصف: |
Background Naturally occurring pre-S deletion mutants have been identified in hepatitis B carriers and shown to be associated with the development of hepatocellular carcinoma. The phenotypes of these pre-S deletion genomes remain unclear, and they were investigated in this study. Methods The pre-S deletion genomes: (1) pre-S1 deletion, (2) deletion spanning pre-S1 and pre-S2, (3) pre-S2 N-terminal deletion, and (4) pre-S2 internal deletion were constructed and analyzed by transfection into Huh-7 cells. Results Functional analyses reveal that these mutants were divided into two groups: S promoter deletion and non-S promoter deletion variants. Compared with the wild-type genome, S promoter deletion variants led to an inverse ratio of pre-S1 mRNA and pre-S2/S mRNA, and intracellular accumulation of surface proteins. An interesting finding is that a small amount of L proteins was detected in the medium from S promoter deletion variant-transfected cells. Non-S promoter deletion variants conversely displayed a wild-type like mRNA and protein pattern. The secretion of surface proteins from non-S promoter deletion variants was inhibited less than from S promoter deletion variant. Immunofluorescence analysis showed mutant surface proteins colocalized with ER and exhibited an atypical distribution: granular staining pattern in the S-promoter deletion variants and perinuclear staining pattern in the non-S promoter deletion variants. Conclusion This study shows that these pre-S deletion genomes exhibit two different phenotypes in mRNA transcription, surface protein expression and secretion. This diversity seems to result from the deletion of S promoter rather than result from the deletion of pre-S1 or pre-S2. |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.jbiomedsci.com/content/19/1/17Test |
| الإتاحة: |
http://www.jbiomedsci.com/content/19/1/17Test |
| حقوق: |
Copyright 2012 Lin et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.E3956D51 |
| قاعدة البيانات: |
BASE |
