التفاصيل البيبلوغرافية
| العنوان: |
Single-cell screening of photosynthetic growth and lactate production by cyanobacteria |
| المؤلفون: |
Hammar, Petter, Angermayr, S., Sjostrom, Staffan, van der Meer, Josefin, Hellingwerf, Klaas, Hudson, Elton, Joensson, Haakan |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2015 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
Cyanobacteria, Single cell, Droplet microfluidics, High-throughput screening, Lactate dehydrogenase |
| الوصف: |
Background Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not feasible. Results We present a method for high-throughput, single-cell analysis and sorting of genetically engineered l -lactate-producing strains of Synechocystis sp. PCC6803. A microfluidic device is used to encapsulate single cells in picoliter droplets, assay the droplets for l -lactate production, and sort strains with high productivity. We demonstrate the separation of low- and high-producing reference strains, as well as enrichment of a more productive l -lactate-synthesizing population after UV-induced mutagenesis. The droplet platform also revealed population heterogeneity in photosynthetic growth and lactate production, as well as the presence of metabolically stalled cells. Conclusions The workflow will facilitate metabolic engineering and directed evolution studies and will be useful in studies of cyanobacteria biochemistry and physiology. |
| نوع الوثيقة: |
other/unknown material |
| اللغة: |
English |
| العلاقة: |
http://www.biotechnologyforbiofuels.com/content/8/1/193Test |
| الإتاحة: |
http://www.biotechnologyforbiofuels.com/content/8/1/193Test |
| حقوق: |
Copyright 2015 Hammar et al. |
| رقم الانضمام: |
edsbas.64730DF |
| قاعدة البيانات: |
BASE |
