دورية أكاديمية
Electrochemical aptasensor array for multiple detection of human osteopontin
| العنوان: | Electrochemical aptasensor array for multiple detection of human osteopontin |
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| المؤلفون: | Meirinho, Sofia G., Dias, L.G., Peres, António M., Rodrigues, Lígia R. |
| سنة النشر: | 2018 |
| المجموعة: | Biblioteca Digital do Instituto Politécnico de Bragança (IPB) |
| مصطلحات موضوعية: | Aptamer, Aptasensor array, Dual-screen-printed gold electrode, Electrochemical detection, Osteopontin |
| الوصف: | Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN) 6 ] 3-/4- as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall. ; The authors acknowledge the financial support from the Strategic funding of UID/BIO/04469/2013 and from project ref. RECI/BBB-EBI/ 0179/2012 (project number FCOMP-01-0124-FEDER-027462). This work was also financially supported by Project POCI-01–0145-FEDER-006984 – Associate Laboratory LSRE-LCM and by Project UID/QUI/00616/2013 – CQ-VR, both funded by FEDER - Fundo Europeu de Desenvolvimento Regional through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia, Portugal. S. Meirinho also acknowledges the doctoral grant (ref SFRH/BD/65021/2009) funded by FCT. ; info:eu-repo/semantics/publishedVersion |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0872-1904 |
| العلاقة: | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F65021%2F2009/PT; info:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04469%2F2013/PT; info:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00616%2F2013/PT; Meirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2018). Electrochemical aptasensor array for multiple detection of human osteopontin. Portugaliae Electrochimica Acta. ISSN 0872-1904. 36, p. 1-9; http://hdl.handle.net/10198/15375Test |
| DOI: | 10.4152/pea.201801001 |
| الإتاحة: | https://doi.org/10.4152/pea.201801001Test http://hdl.handle.net/10198/15375Test |
| حقوق: | openAccess |
| رقم الانضمام: | edsbas.A4CEA83 |
| قاعدة البيانات: | BASE |
| تدمد: | 08721904 |
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| DOI: | 10.4152/pea.201801001 |