دورية أكاديمية
Simultaneous quantification of Gemcitabine and Irinotecan hydrochloride in rat plasma by using high performance liquid chromatography-diode array detector
| العنوان: | Simultaneous quantification of Gemcitabine and Irinotecan hydrochloride in rat plasma by using high performance liquid chromatography-diode array detector |
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| المؤلفون: | Malatesta Luciano, Cosco Donato, Paolino Donatella, Cilurzo Felisa, Costa Nicola, Di Tullio Alessandra, Fresta Massimo, Celia Christian, Di Marzio Luisa, Locatelli Marcello |
| المساهمون: | Malatesta, Luciano, Cosco, Donato, Paolino, Donatella, Cilurzo, Felisa, Costa, Nicola, Di Tullio, Alessandra, Fresta, Massimo, Celia, Christian, DI MARZIO, Luisa, Locatelli, Marcello |
| سنة النشر: | 2018 |
| المجموعة: | ARUd'A - Archivio Istituzionale della ricerca dell'università Chieti-Pescara (IRIS) |
| مصطلحات موضوعية: | HPLC-DAD method validation, Sprague Dawley rat plasma and sample preparation, biological samples, Gemcitabine and Irinotecan hydrochloride association, pharmacokinetic analysis |
| الوصف: | In this manuscript we aimed at the simultaneous separation and quantification of Gemcitabine and Irinotecan hydrochloride (injected both as single components and in combination) from Sprague Dawley rat plasma by using a validated method obtained through the use of a High Performance Liquid Chromatography (HPLC)-diode array detector (DAD). Gemcitabine and Irinotecan hydrochloride were detected and quantified using a Zorbax Extend C-18 column (250 mm × 4.6 mm; 5 m particle size) in gradient elution mode. The chromatographic analyses were carried out in 15 minutes. The analytical mode was calibrated and validated in the concentration range from 0.1 to 18 g/mL both for Gemcitabine and Irinotecan hydrochloride. Sprague Dawley rat plasma was used to perform the analysis. 3-methylxanthine was the internal standard. The weighted-matrix matched standard curves of Gemcitabine and Irinotecan hydrochloride showed a good linearity up to 18 g/mL. Parallelism tests were also performed to evaluate whether the over-range samples could be analyzed after dilution without affecting the analytical performance. The intra- and inter-day precision (RSD%) values of Gemcitabine and Irinotecan hydrochloride were ≤7.14% and ≤11.5%, respectively. The intra- and inter-day trueness (Bias%) values were in the range from -11.5% to 1.70% for both drugs. The analytical mode performance was further tested after collecting Sprague Dawley rat plasma following a single-dose administration of chemotherapeutics or their association. The validated HPLC-DAD method allowed the simultaneous quantification of Gemcitabine and Irinotecan hydrochloride in the rat plasma, besides the evaluation of the pharmacokinetic parameters and drug delivery. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/29990886; info:eu-repo/semantics/altIdentifier/wos/WOS:000442699900022; volume:159; firstpage:192; lastpage:199; numberofpages:8; journal:JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS; http://hdl.handle.net/11564/694049Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85049466203 |
| DOI: | 10.1016/j.jpba.2018.06.060 |
| الإتاحة: | https://doi.org/10.1016/j.jpba.2018.06.060Test http://hdl.handle.net/11564/694049Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.12DF0FC4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jpba.2018.06.060 |
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