دورية أكاديمية
Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A 1 Receptor Ligands
| العنوان: | Multipotent Stromal Cells from Subcutaneous Adipose Tissue of Normal Weight and Obese Subjects: Modulation of Their Adipogenic Differentiation by Adenosine A 1 Receptor Ligands |
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| المؤلفون: | Zuccarini M., Lambertucci C., Carluccio M., Giuliani P., Ronci M., Spinaci A., Volpini R., Ciccarelli R., Di Iorio P. |
| المساهمون: | Zuccarini, M., Lambertucci, C., Carluccio, M., Giuliani, P., Ronci, M., Spinaci, A., Volpini, R., Ciccarelli, R., Di Iorio, P. |
| سنة النشر: | 2021 |
| المجموعة: | ARUd'A - Archivio Istituzionale della ricerca dell'università Chieti-Pescara (IRIS) |
| مصطلحات موضوعية: | Adenosine A, receptor, Adipogenic differentiation, Adipogenic marker, Adipose stromal cells (ASCs), Subcutaneous adipose tissue, Adenosine A1 Receptor Agonist, Adult, Apoptosi, Biomarker, Cell Proliferation, Female, Human, Ligand, Mesenchymal Stem Cell, Necrosi, Obesity, Phosphatidylinositol 3-Kinase, Phosphorylation, Proto-Oncogene Proteins c-akt, Adenosine A1, Signal Transduction, Subcutaneous Fat, Adipogenesi, Body Weight |
| الوقت: | 1 |
| الوصف: | Adenosine A1 receptor (A1R) activation, stimulating lipogenesis and decreasing insulin resistance, could be useful for metabolic syndrome management in obese subjects. Since full A1R agonists induce harmful side-effects, while partial agonists show a better pharmacological profile, we investigated the influence of two derivatives of the full A1R agonist 2-chloro-N6-cyclopentyladenosine (CCPA), C1 and C2 behaving as A1R partial agonists in animal models, on the adipogenic differentiation of stromal/stem cells (ASCs) from human subcutaneous adipose tissue, which mainly contribute to increase fat mass in obesity. The ASCs from normal-weight subjects showed increased proliferation and A1R expression but reduced adipogenic differentiation compared to obese individual-derived ASCs. Cell exposure to CCPA, C1, C2 or DPCPX, an A1R antagonist, did not affect ASC proliferation, while mainly C2 and DPCPX significantly decreased adipogenic differentiation of both ASC types, reducing the activity of glycerol-3-phosphate dehydrogenase and the expression of PPARγ and FABP-4, all adipogenic markers, and phosphorylation of Akt in the phosphatidylinositol-3-kinase pathway, which plays a key-role in adipogenesis. While requiring confirmation in in vivo models, our results suggest that A1R partial agonists or antagonists, by limiting ASC differentiation into adipocytes and, thereby, fat mass expansion, could favor development/worsening of metabolic syndrome in obese subjects without a dietary control. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34944069; info:eu-repo/semantics/altIdentifier/wos/WOS:000736248200001; volume:10; issue:12; firstpage:3560; journal:CELLS; http://hdl.handle.net/11564/773831Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85121294521; https://www.mdpi.com/2073-4409/10/12/3560Test |
| DOI: | 10.3390/cells10123560 |
| الإتاحة: | https://doi.org/10.3390/cells10123560Test http://hdl.handle.net/11564/773831Test https://www.mdpi.com/2073-4409/10/12/3560Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B4ED15F4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cells10123560 |
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