دورية أكاديمية
Binding of model polycyclic aromatic hydrocarbons and carbamate-pesticides to DNA, BSA, micelles and liposomes
| العنوان: | Binding of model polycyclic aromatic hydrocarbons and carbamate-pesticides to DNA, BSA, micelles and liposomes |
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| المؤلفون: | Macii F., Salvadori G., BONINI, RACHELE, Giannarelli S., Mennucci B., Biver T. |
| المساهمون: | Macii, F., Salvadori, G., Bonini, Rachele, Giannarelli, S., Mennucci, B., Biver, T. |
| سنة النشر: | 2019 |
| المجموعة: | ARPI - Archivio della Ricerca dell'Università di Pisa |
| مصطلحات موضوعية: | Intercalation, Persistent organic pollutants (POPs), Quantum mechanic, Spectrofluorometry, Spectrophotometry, TD(DFT) |
| الوصف: | The binding to biosubstrates and micellar systems of pollutants as the polycyclic aromatic hydrocarbon (PAH) derivatives 1-aminopyrene (1-PyNH2) and 1-hydroxymethylpyrene (1-PyMeOH) and the carbamate-pesticides 1-naphthyl-N-methylcarbamate (carbaryl, CA) and methyl benzimidazol-2-ylcarbamate (carbendazim, CBZ) was analysed through an integrated strategy combining spectroscopy and quantum chemistry. As biosubstrates, natural DNA and bovine serum albumin (BSA) were taken into account for a thermodynamic analysis of the binding features through spectrophotometric and spectrofluorometric techniques. In all cases, a strong DNA interaction is present and intercalation is supposed as the major binding mode. For the PAH derivatives, DNA binding is found to be favoured under high salt conditions and BSA static quenching and binding with 1:1 stoichiometry occurs. The molecular structure and optical properties of 1-PyNH2, CA and CBZ together with their intercalated adducts in DNA were studied also by means of quantum chemical approach. The (TD)DFT calculations on intercalated dye/DNA adducts quantitatively reproduce the experimentally observed spectroscopic changes, thus confirming the intercalation hypothesis. The theoretical approach also provides information on the adducts' geometries and on the amount of charge transfer with DNA. Moreover, ultrafiltration tests in the presence of anionic (SDS), cationic (DTAC) and neutral (Triton X) micellar aggregates and liposomes provided insights into lipophilicity and cellular membrane affinity. PAH derivatives show high retention coefficient in all cases, whereas in the case of carbamate-pesticides micellar retention might be significantly reduced and is very limited in the case of liposomes. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31277031; info:eu-repo/semantics/altIdentifier/wos/WOS:000489293900025; volume:223; numberofpages:10; journal:SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; http://hdl.handle.net/11568/995527Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85068195183; https://www.sciencedirect.com/science/article/pii/S1386142519307036Test |
| DOI: | 10.1016/j.saa.2019.117313 |
| الإتاحة: | https://doi.org/10.1016/j.saa.2019.117313Test http://hdl.handle.net/11568/995527Test https://www.sciencedirect.com/science/article/pii/S1386142519307036Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.905E0054 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.saa.2019.117313 |
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