دورية أكاديمية
Lamivudine-resistant HBV strain rtM204V/I in acute hepatitis B
| العنوان: | Lamivudine-resistant HBV strain rtM204V/I in acute hepatitis B |
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| المؤلفون: | Sagnelli, E, Sagnelli, C, Alessio, L, Moriconi, F, Messina, V, Pisaturo, M, Colombatto, P, Tonziello, G, Coppola, N., BRUNETTO, MAURIZIA ROSSANA, CAVALLONE, DANIELA |
| المساهمون: | Sagnelli, E, Brunetto, MAURIZIA ROSSANA, Cavallone, Daniela, Sagnelli, C, Alessio, L, Moriconi, F, Messina, V, Pisaturo, M, Colombatto, P, Tonziello, G, Coppola, N. |
| سنة النشر: | 2013 |
| المجموعة: | ARPI - Archivio della Ricerca dell'Università di Pisa |
| الوصف: | AIMS: To detect HBV rtM204V/I lamivudine-resistant strains in serum of patients with acute hepatitis B and to assess their biological and clinical significance. METHODS: Eighty HBV DNA-positive patients with symptomatic acute hepatitis B observed from 1999 to 2010 were enrolled. A plasma sample obtained at the first observation was tested for HBV mutants in the polymerase region by direct sequencing; the antiviral drug-resistant rtM204V/I mutations, the most frequent HBV mutants in Italy, were also sought by the more sensitive allele-specific polymerase chain reaction (PCR). RESULTS: No HBV mutation associated with resistance to nucleos(t)ide analogues was identified by direct sequencing, whereas allele-specific PCR identified HBV strains carrying the substitution rtM204V/I in 11 (13.7%) patients. Compared with those with the HBV wild strain, patients with rtM204V/I more frequently showed severe acute hepatitis B (36.4% vs 8.7%; p < 0.05) and lower values of serum HBV DNA (1.77 × 10(6) ± 4.76 × 10(6) vs. 1.68 × 10(8) ± 5.46 × 10(8)). In addition, a multivariate analysis identified the presence of a pre-existing HCV chronic infection as independently associated with severe acute hepatitis B (p < 0.05). CONCLUSIONS: HBV rtM204V/I lamivudine-resistant strains were detected in serum of 11 (13.7%) patients with acute hepatitis B by allele-specific polymerase chain reaction. The frequent association of rtM204V/I with a more severe acute hepatitis B and with a lower viral load may suggest that greater and/or more prolonged immune pressure might have induced their selection. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/23796869; volume:67; issue:4; firstpage:322; lastpage:328; numberofpages:7; journal:JOURNAL OF INFECTION; http://hdl.handle.net/11568/844235Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84882838835 |
| DOI: | 10.1016/j.jinf.2013.06.006 |
| الإتاحة: | https://doi.org/10.1016/j.jinf.2013.06.006Test http://hdl.handle.net/11568/844235Test |
| رقم الانضمام: | edsbas.E97436CD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jinf.2013.06.006 |
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