دورية أكاديمية
Neuropeptide S promotes wakefulness through the inhibition of sleep-promoting VLPO neurons
| العنوان: | Neuropeptide S promotes wakefulness through the inhibition of sleep-promoting VLPO neurons |
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| المؤلفون: | Chauveau, Frédéric, Claverie, Damien, Lardant, Emma, Varin, Christophe, Hardy, Eléonore, Walter, Augustin, Rouach, Nathalie, Canini, Frédéric, Rancillac, Armelle |
| المساهمون: | Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA), Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Neurophysiology Brussels, Université libre de Bruxelles (ULB), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École du Val de Grâce (EVDG), Service de Santé des Armées |
| المصدر: | ISSN: 0161-8105. |
| بيانات النشر: | HAL CCSD Oxford University Press |
| سنة النشر: | 2019 |
| المجموعة: | Archive ouverte du Service de Santé des Armées (HAL) |
| مصطلحات موضوعية: | neurovascular coupling, feed-forward inhibition, VLPO, NREM sleep, NPS, GABAergic neurons, patch-clamp, polysomnography, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] |
| الوصف: | Nathalie Rouach and Frédéric Canini contributed equally to this work. ; International audience ; STUDY OBJECTIVES:The regulation of sleep-wake cycles is crucial for the brain's health and cognitive skills. Among the various substances known to control behavioral states, intraventricular injection of neuropeptide S (NPS) has already been shown to promote wakefulness. However, the NPS signaling pathway remains elusive. In this study, we characterized the effects of NPS in the ventrolateral preoptic nucleus (VLPO) of the hypothalamus, one of the major brain structures regulating non-rapid eye movement (NREM) sleep.METHODS:We combined polysomnographic recordings, vascular reactivity, and patch-clamp recordings in mice VLPO to determine the NPS mode of action.RESULTS:We demonstrated that a local infusion of NPS bilaterally into the anterior hypothalamus (which includes the VLPO) significantly increases awakening and specifically decreases NREM sleep. Furthermore, we established that NPS application on acute brain slices induces strong and reversible TTX-sensitive constriction of blood vessels in the VLPO. This effect strongly suggests that the local neuronal network is downregulated in the presence of NPS. At the cellular level, we revealed by electrophysiological recordings and in situ hybridization that NPSR mRNAs are only expressed by non-Gal local GABAergic neurons, which are depolarized by the application of NPS. Simultaneously, we showed that NPS hyperpolarizes sleep-promoting neurons, which is associated with an increased frequency in their spontaneous IPSC inputs.CONCLUSION:Altogether, our data reveal that NPS controls local neuronal activity in the VLPO. Following the depolarization of local GABAergic neurons, NPS indirectly provokes feed-forward inhibition onto sleep-promoting neurons, which translates into a decrease in NREM sleep to favor arousal. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31403694; inserm-02267450; https://inserm.hal.science/inserm-02267450Test; https://inserm.hal.science/inserm-02267450/documentTest; https://inserm.hal.science/inserm-02267450/file/Chauveau%20et%20al,%202019.pdfTest; PUBMED: 31403694 |
| DOI: | 10.1093/sleep/zsz189 |
| الإتاحة: | https://doi.org/10.1093/sleep/zsz189Test https://inserm.hal.science/inserm-02267450Test https://inserm.hal.science/inserm-02267450/documentTest https://inserm.hal.science/inserm-02267450/file/Chauveau%20et%20al,%202019.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.EB8B20A0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/sleep/zsz189 |
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