دورية أكاديمية
Age related acute effects of microcystin-LR on Daphnia magna biotransformation and oxidative stress
| العنوان: | Age related acute effects of microcystin-LR on Daphnia magna biotransformation and oxidative stress |
|---|---|
| المؤلفون: | Ortiz-Rodriguez, Rafael, Wiegand, Claudia |
| المساهمون: | Leibniz Institute of Freshwater Ecology & Inland Fisheries, Ecosystèmes, biodiversité, évolution Rennes (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), University of Southern Denmark (SDU) |
| المصدر: | ISSN: 0041-0101. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2010 |
| المجموعة: | Archive Ouverte de l'Université Rennes (HAL) |
| مصطلحات موضوعية: | Cyanobacteria, Microcystin-LR, Antioxidant enzymes, Oxidative stress, Biotransformation, Lactate dehydrogenase, [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
| الوصف: | International audience ; As a result of eutrophication among other factors, bloom forming species of cyanobacteria frequently thrive and dominate communities in freshwater ecosystems. Cyanobacteria are considered as a nuisance and hazardous due to the production of toxins. The most potent and studied cyanobacterial toxins are the microcystins, especially the variant microcystin-LR (MC-LR). Daphnia magna plays a central role in freshwater pelagic food webs and its populations are negatively affected by cyanobacteria due to nutritional inadequacy, feeding interference and toxic effects on growth and reproduction. Biotransformation and antioxidant enzymes, amongst others enable D. magna capacity to cope with MC-LR up to a certain concentration. Aim of our study was to investigate the age related acute effects of MC-LR on D. magna biotransformation and antioxidant enzymes (glutathione S-transferase, GST and catalase, CAT), subsequent lipid peroxidation and furthermore to lactate and the enzyme lactate dehydrogenase (LDH). D. magna neonates (<3 days old) and young adults (7 days old), were exposed to increasing concentrations of MC-LR (up to 100 mg/L) for 24 and 48 h. D. magna sGST activities at both ages, but more pronounced in adults, increased at low MC-LR concentrations followed by decreased activities at the higher ones suggesting diminished ability to biotransform the cyanotoxin. Oxidative protection was more efficient in neonates, where CAT was elevated strongly and contributed to buffer oxidative stress, evidenced by the constant TBARS concentrations. LDH was negatively affected by MC-LR exposure leading to constant and low concentrations of lactate. As thisenzyme is involved in the production of rapidly required energy, the findings suggest an energetic impairment due to MC-LR. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01120453; https://univ-rennes.hal.science/hal-01120453Test |
| الإتاحة: | https://univ-rennes.hal.science/hal-01120453Test |
| رقم الانضمام: | edsbas.786A778F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |