دورية أكاديمية
High proliferation and delamination during skin epidermal stratification.
| العنوان: | High proliferation and delamination during skin epidermal stratification. |
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| المؤلفون: | Damen, Mareike, Wirtz, Lisa, Soroka, Ekaterina, Khatif, Houda, Kukat, Christian, Simons, Benjamin D, Bazzi, Hisham |
| المصدر: | essn: 2041-1723 ; nlmid: 101528555 |
| بيانات النشر: | Nature communications |
| سنة النشر: | 2021 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Epidermis, Hair Follicle, Centrosome, Keratinocytes, Animals, Mice, Knockout, Humans, Ubiquitin Thiolesterase, Cell Differentiation, Cell Proliferation, Models, Biological, Adolescent, Adult, Aged, Middle Aged, Child, Preschool, Female, Male, Tumor Suppressor Protein p53, Embryo, Mammalian, Skin Physiological Phenomena, Young Adult, Time-Lapse Imaging, Asymmetric Cell Division, Spindle Apparatus, Tumor Suppressor p53-Binding Protein 1 |
| الوصف: | The development of complex stratified epithelial barriers in mammals is initiated from single-layered epithelia. How stratification is initiated and fueled are still open questions. Previous studies on skin epidermal stratification suggested a central role for perpendicular/asymmetric cell division orientation of the basal keratinocyte progenitors. Here, we use centrosomes, that organize the mitotic spindle, to test whether cell division orientation and stratification are linked. Genetically ablating centrosomes from the developing epidermis leads to the activation of the p53-, 53BP1- and USP28-dependent mitotic surveillance pathway causing a thinner epidermis and hair follicle arrest. The centrosome/p53-double mutant keratinocyte progenitors significantly alter their division orientation in the later stages without majorly affecting epidermal differentiation. Together with time-lapse imaging and tissue growth dynamics measurements, the data suggest that the first and major phase of epidermal development is boosted by high proliferation rates in both basal and suprabasally-committed keratinocytes as well as cell delamination, whereas the second phase maybe uncoupled from the division orientation of the basal progenitors. The data provide insights for tissue homeostasis and hyperproliferative diseases that may recapitulate developmental programs. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/324614Test |
| DOI: | 10.17863/CAM.72069 |
| الإتاحة: | https://doi.org/10.17863/CAM.72069Test https://www.repository.cam.ac.uk/handle/1810/324614Test |
| حقوق: | Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.3460F4B4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.72069 |
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