التفاصيل البيبلوغرافية
| العنوان: |
The immunomodulatory peptide bursopentin (BP5) enhances proliferation and induces sIgM expression in DT40 cells |
| المؤلفون: |
Ji, Xiang-Bo, Luo, Jun, Feng, Xiu-Li, Xu, Qiu-Liang, Man, Teng, Zhao, Dong, Li, Xin-Feng, Zhang, Gai-Ping, Chen, Pu-Yan |
| المصدر: |
African Health Sciences; Vol 18, No 4 (2018); 1292-1302 ; 1680-6905 |
| بيانات النشر: |
Makerere University Medical School (Uganda) |
| سنة النشر: |
2018 |
| المجموعة: |
AJOL - African Journals Online |
| مصطلحات موضوعية: |
Bursopentin (BP5), DT40 cell, Proliferation, Cell cycle, sIgM, gene microarray |
| الوصف: |
Background: In the recent past, many studies have been focused on extracts of BF and multiple biologically active factors and their effects on humoral immune system in chickens and birds. However, the mechanism of those immunomodulatory peptides on the B lineage cells proliferation and antibody production in chicken is fairly unknown. DT40 cell line, an avian leucosis virus-induced chicken pre-B cell line, expresses immunoglobulin M (IgM) isotype B cell reporter in the plasma membrane. There are many evidences suggesting that DT40 cells are best characterized as a bursal stem cell line. Because of the unique characteristics of DT40 cell line, it has been widely used to observe biological processes of pre-B lymphocyte cell within living cells.Methods: The chicken B cell line DT40 was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium and cytotoxicity was studied. Also, effect of BP5 on cell proliferation and cell cycle distribution of DT40 cells was studied. Also, the effect of BP5 on sIgM mRNA expression was studied by using real-time PCR. Objectives: To investigat the effects of Bursopentin (Cys-Lys-Arg-Val-Tyr, BP5) on a chicken promyelocyte cell line DT40, assays of cell proliferation, cell cycle distribution, detection of surface immunoglobulin G (sIgM) mRNA expression and gene microarray analysis were performed. Results: The results showed that BP5 displayed concentration-dependent effects on the proliferation, cell cycle, and sIgM mRNA expression in DT40 cells. And the analysis of expression profiles identified a signature set of 3022 genes (1254 up regulated genes, 1762 down regulated genes), which clearly discriminated the BP5-treated DT40 cells from control with high certainty (P≤0.02). The results of microarray analysis were confirmed by quantitative reverse transcription-polymerase chain reaction for 12 of the differentially expressed genes. Conclusion: Theses findings showed the immuno-activity effect of BP5 on B lymphocyte and indicated that BP5 treatment regulated eight signaling ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
https://www.ajol.info/index.php/ahs/article/view/180342/169689Test; https://www.ajol.info/index.php/ahs/article/view/180342Test |
| الإتاحة: |
https://www.ajol.info/index.php/ahs/article/view/180342Test |
| حقوق: |
A. AFRICAN HEALTH SCIENCES OPEN ACCESS POLICYWhile African Health Sciences has been freely accessible online there have been questions on whether it is Open Access or not. We wish to clearly state that indeed African Health Sciences is Open Access. There are key issues regarding Open Access needing clarification for avoidance of doubt:1. Henceforth, papers in African Health Sciences will be published under the CC BY (Creative Commons Attribution License) 4.0 International. See details on https://creativecomons.orgTest/)2. The copyright owners or the authors grant the 3rd party (perpetually and in advance) the right to disseminate, reproduce, or use the research papers in part or in full, format/medium as long as:No substantive errors are introduced in the processAttribution of authorship and correct citation details are givenThe referencing details are not changed. Should the papers be reproduced in part, this must be clearly stated.3. The papers will be freely and universally accessible online in an easily readable format such as XML in at least one widely recognized open access repository such as PUBMED CENTRAL.B. ABRIDGED LICENCE AGREEMENT BETWEEN AUTHORS AND African Health SciencesI submitted my manuscript to African Health Sciences and would like to affirm that: 1.0 I am authorized by my co-authors to enter into these arrangements.2.0 I guarantee, on behalf of self and co-authors:That the paper is original, and has not been published in any other peer-reviewed journal; nor is it under consideration by other journal (s). It does not infringe existing copyright or any other person’s rights That we are/I am the sole author(s) of the paper and with authority to enter into this agreement. My granting rights to African Health Sciences is not in breach of any other obligation That the paper contains nothing unlawful, or libelous. Nor anything that would constitute a breach of contract, confidence or commitment given to secrecy, if published That I/we have taken care to ensure the integrity of the article.3.0 I and all co-authors, agree that the paper, if accepted for publication, shall be licensed under the Creative Commons Attribution License 4.0. (see https://creativecommons.orgTest/) |
| رقم الانضمام: |
edsbas.E4586638 |
| قاعدة البيانات: |
BASE |
