دورية أكاديمية
Symptom Severity of Nicotiana benthamiana Plants Inoculated with Agrobacterium Containing Infectious DNA-A Clones of Honeysuckle Yellow Vein Virus (HYVV)
| العنوان: | Symptom Severity of Nicotiana benthamiana Plants Inoculated with Agrobacterium Containing Infectious DNA-A Clones of Honeysuckle Yellow Vein Virus (HYVV) |
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| المؤلفون: | Oh, Sung, Choi, Chang Won |
| المصدر: | International Annals of Science; Vol. 7 No. 1 (2019); 12-20 ; 2456-7132 ; 10.21467/ias.7.1.2019 |
| بيانات النشر: | AIJR Publisher |
| سنة النشر: | 2019 |
| المجموعة: | Advanced International Journals of Research (AIJR) |
| مصطلحات موضوعية: | Honeysuckle yellow vein virus (HYVV), Symptom severity, Virulence of HYVV DNA-A clones, V1 protein gene, SYBR Green-based real-time PCR |
| الوصف: | To investigate the pathogenicity and virulence of the Honeysuckle yellow vein virus (HYVV) lacking betasatellites, PCR amplified unit-lengths of DNA-A genome of HYVV-[DJ] were cloned into binary vector pRI101-AN, and generated HYVV-[DJ]-1mer, -1.3mer and -2mer genomes. Each construct was transformed into Agrobacterium cells and agro-inoculated into young leaves of Nicotiana benthamiana. Except for the HYVV-[DJ]-1mer, HYVV-[DJ]-1.3mer and -2mer clones caused pronounced disease symptoms in N. benthamiana. HYVV-[DJ]-2mer agro-inoculated plants showed more severe plant stunting with downward leaf curling and crinkling than those of HYVV-[DJ]-1.3mer agro-inoculated plants. To discriminate the clone’s virulence quantitatively, SYBR Green-based real-time PCR was performed for the quantification of the target virulence gene DNA in agro-inoculated plants that were collected at weekly intervals for 4 weeks. Regression analysis was obtained from the standard curves by plotting Ct values over the logarithm of the amount of V1 protein gene DNA present in a dilution series of plasmid containing the full-length HYVV-[DJ] genome. Equation of the HYVV V1 DNA standard curve was used to quantify V1 gene DNA concentration in agro-inoculated plants with each clone. The accumulation of V1 gene DNA in HYVV-[DJ]-1.3mer agro-inoculated plants reached the peak level at 4 weeks post inoculation, while the accumulation of V1 gene DNA in HYVV-[DJ]-2mer agro-inoculated plants reached the peak level at 3 weeks post inoculation. The amount of V1 DNA in HYVV-[DJ]-1.3mer agro-inoculated plants was significantly more than that in HYVV-[DJ]-2mer agro-inoculated plants. Considering the results, there was a difference between the accumulation of virus DNA and the symptom severity of the analyzed plants agro-inoculated with each clone. It suggested that the infectious clones’ virulence is not necessarily correlated with the symptom severity. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://journals.aijr.org/index.php/ias/article/view/1390/206Test; https://journals.aijr.org/index.php/ias/article/view/1390Test |
| الإتاحة: | https://doi.org/10.21467/ias.7.1.2019Test https://journals.aijr.org/index.php/ias/article/view/1390Test |
| حقوق: | Copyright (c) 2019 Sung Oh, Chang Won Choi |
| رقم الانضمام: | edsbas.3299BD6A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |