دورية أكاديمية
The potential of local orange peel-derived eco-enzymes in producing indole acetic acid
العنوان: | The potential of local orange peel-derived eco-enzymes in producing indole acetic acid |
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المؤلفون: | Farma, Siska Alicia, Luzik, Nurfa Dewiza, Sakina, Salma, Putri, Irma Leilani Eka, Advinda, Linda, Anhar, Azwir |
المصدر: | Acta Biochimica Indonesiana; Vol. 6 No. 2 (2023): Acta Biochimica Indonesiana; 135 ; 2654-3222 ; 2654-6108 |
بيانات النشر: | Indonesian Society for Biochemistry and Molecular Biology |
سنة النشر: | 2023 |
المجموعة: | Acta Biochimica Indonesiana |
مصطلحات موضوعية: | Ecoenzyme, Indole Acetic Acid, Orange Peel, Sustainable Agriculture, Phytohormones |
الوصف: | Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture. Objective: This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones. Methods: The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method. Results: ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL). Conclusion: Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture. |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
العلاقة: | http://jurnal.pbbmi.org/index.php/actabioina/article/view/135/71Test; http://jurnal.pbbmi.org/index.php/actabioina/article/view/135Test |
DOI: | 10.32889/actabiona.137 |
الإتاحة: | https://doi.org/10.32889/actabiona.137Test http://jurnal.pbbmi.org/index.php/actabioina/article/view/135Test |
حقوق: | Copyright (c) 2023 Authors ; https://creativecommons.org/licenses/by-nc/4.0Test |
رقم الانضمام: | edsbas.557A7411 |
قاعدة البيانات: | BASE |
DOI: | 10.32889/actabiona.137 |
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