دورية أكاديمية
Cloning and expression of Plasmodium falciparum lactate dehydrogenase (PfLDH) in Escherichia coli BL21(DE3)
| العنوان: | Cloning and expression of Plasmodium falciparum lactate dehydrogenase (PfLDH) in Escherichia coli BL21(DE3) |
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| المؤلفون: | Masduki, Fifi Fitriyah, Hotimah, Yeni, Rani, Rosalia, Mawardi, Arsyam, Ramandey, Euniche R.P.F., Fibriani, Azzania, Suhandono, Sony |
| المصدر: | Acta Biochimica Indonesiana; Vol. 2 No. 2 (2019): Acta Biochimica Indonesiana; 52-57 ; 2654-3222 ; 2654-6108 |
| بيانات النشر: | Indonesian Society for Biochemistry and Molecular Biology |
| سنة النشر: | 2019 |
| المجموعة: | Acta Biochimica Indonesiana |
| مصطلحات موضوعية: | Plasmodium falciparum, lactate dehydrogenase, Malaria Rapid Diagnostic Test (RDT), recombinant protein |
| الوصف: | Background:Immediate and accurate diagnosis of malaria is essential for effective control of this disease. Immunochromatographic based rapid diagnostic tests (RDTs) are economical, simple to perform, and provide results in a relative short time, can be useful to assist effective management of malaria. The commercially available malaria RDT in Indonesia is still imported. Therefore, an effort to produce malaria RDT independently is necessary. One of the biomarkers used in RDTs isPlasmodiumlactate dehydrogenase pLDH. The production and accumulation of pLDH during asexual stage or blood-stage in all human infected malaria parasites can be used to indicate parasites viability, which is correlated with the number of parasites present in the plasma of infected patients. Objective: The aim of this research is to produce recombinant PfLDH inEscherichia coliBL21(DE3). Methods:PfLDH gene was cloned into pET30a expression vector to obtain a 6.2 kbp recombinant plasmid pET30a-PfLDH.E. coliBL21(DE3) was transformed with pET30a-PfLDH using the heat shock method. Then,E. coliBL21(DE3)- pET30a-PfLDH was cultured in LB broth containing 50 mg/mLkanamycin and was induced by 1mM IPTG at 37oC. Results: SDS-PAGE and Western Blot analysis showed that recombinantPfLDH was expressed with molecular mass ~30 kDa. Conclusion: RecombinantPfLDH is expressed inE. coliBL21(DE3) and can be used in further research for producing rPfLDH as a biomarker for malaria RDT development. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://jurnal.pbbmi.org/index.php/actabioina/article/view/41/27Test; http://jurnal.pbbmi.org/index.php/actabioina/article/view/41Test |
| DOI: | 10.32889/actabioina.v2i2.39 |
| الإتاحة: | https://doi.org/10.32889/actabioina.v2i2.39Test http://jurnal.pbbmi.org/index.php/actabioina/article/view/41Test |
| حقوق: | Copyright (c) 2019 Authors ; https://creativecommons.org/licenses/by-nc/4.0Test |
| رقم الانضمام: | edsbas.E341DBCD |
| قاعدة البيانات: | BASE |
| DOI: | 10.32889/actabioina.v2i2.39 |
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