دورية أكاديمية
Cellular distribution, regulated expression, and functional role of the Anorexigenic Peptide, NUCB2/Nesfatin-1, in the testis
| العنوان: | Cellular distribution, regulated expression, and functional role of the Anorexigenic Peptide, NUCB2/Nesfatin-1, in the testis |
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| المؤلفون: | Garcia-Galiano, David, Pineda, Rafael, Castellano, Juan Manuel, Ruiz-Pino, Francisco, Sánchez-Garrido, Miguel Ángel, Vazquez, Maria J., Sangiao-Alvarellos, Susana, Romero-Ruiz, Antonio, Pinilla, Leonor, Dieguez, Carlos, Gaytan, Francisco, Tena-Sempere, Manuel |
| المساهمون: | Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı., İlhan, Tuncay, AAH-8859-2021, 16549312600 |
| بيانات النشر: | Endocrine Society |
| سنة النشر: | 2012 |
| المجموعة: | Açık Erişim@BUU (Bursa Uludağ Üniversitesi) |
| مصطلحات موضوعية: | Endocrinology & metabolism, Ribonucleic-acid expression, Energy-balance, Rat testis, Paraventricular nucleus, Testosterone secretion, Insulin-secretion, Satiety molecule, Leydig-cells, Orexin-a, Nesfatin-1, Aging, Animals, Calcium-binding proteins, Dna-binding proteins, Energy metabolism, Gene expression regulation, Humans, Leydig cells, Male, Mice, inbred c57bl, Models, animal, Nerve tissue proteins, Rats, wistar, Sexual maturation, Testis, Testosterone |
| الوصف: | Nesfatin-1, product of the precursor NEFA/nucleobindin2 (NUCB2), was initially identified as anorectic hypothalamic neuropeptide, acting in a leptin-independent manner. In addition to its central role in the control of energy homeostasis, evidence has mounted recently that nesfatin-1 is also produced in peripheral metabolic tissues, such as pancreas, adipose, and gut. Moreover, nesfatin-1 has been shown to participate in the control of body functions gated by whole-body energy homeostasis, including puberty onset. Yet, whether, as is the case for other metabolic neuropeptides, NUCB2/nesfatin-1 participates in the direct control of gonadal function remains unexplored. Wedocument here for the first time the expression ofNUCB2mRNAin rat, mouse, and human testes, where NUCB2/nesfatin-1 protein was identified in interstitial mature Leydig cells. Yet in rats, NUCB2/nesfatin-1becameexpressed in Sertoli cellsuponLeydig cell eliminationandwas also detected in Leydig cell progenitors. Although NUCB2 mRNA levels did not overtly change in rat testis during pubertal maturation and after short-term fasting, NUCB2/nesfatin-1 content significantly increased along the puberty-to-adult transition and was markedly suppressed after fasting. In addition, testicular NUCB2/nesfatin-1 expression was up-regulated by pituitary LH, because hypophysectomy decreased, whereas human choriogonadotropin (super-agonist of LH receptors) replacement enhanced, NUCB2/nesfatin-1 mRNA and peptide levels. Finally, nesfatin-1 increased human choriogonadotropin-stimulated testosterone secretion by rat testicular explants ex vivo. Our data are the first to disclose the presence and functional role of NUCB2/nesfatin-1 in the testis, where its expression is regulated by developmental, metabolic, and hormonal cues as well as by Leydig cell-derived factors. Our observations expand the reproductive dimension of nesfatin-1, which may operate directly at the testicular level to link energy homeostasis, puberty onset, and gonadal function. ; Spanish Government - ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0013-7227 1945-7170 22334726 |
| العلاقة: | Makale - Uluslararası Hakemli Dergi; Endocrinology; Yurt dışı; Sanayi; Garcia-Galiano, D. vd. (2012). "Cellular distribution, regulated expression, and functional role of the Anorexigenic Peptide, NUCB2/Nesfatin-1, in the testis". Endocrinology, 153(4), 1959-1971.; https://doi.org/10.1210/en.2011-2032Test; https://academic.oup.com/endo/article/153/4/1959/2424076Test; http://hdl.handle.net/11452/24726Test; 000302169800043; 2-s2.0-84859416910; 1959; 1971; 153 |
| DOI: | 10.1210/en.2011-2032 |
| الإتاحة: | https://doi.org/10.1210/en.2011-2032Test http://hdl.handle.net/11452/24726Test https://academic.oup.com/endo/article/153/4/1959/2424076Test |
| حقوق: | info:eu-repo/semantics/openAccess ; Atıf Gayri Ticari Türetilemez 4.0 Uluslararası ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.A8BEF7D5 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00137227 19457170 22334726 |
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| DOI: | 10.1210/en.2011-2032 |