التفاصيل البيبلوغرافية
| العنوان: |
Gas-liquid mixing in a stirred tank bioreactor with gas-exchange means ; STR bioreaktorin kaasun vaihto tarkastelu kaasu-neste sekoituksessa |
| المؤلفون: |
Qian, Franco |
| المساهمون: |
Lee, Cheng-Kang, Mou, Duen-Gang, Kemian tekniikan korkeakoulu, Bankar, Sandip, Aalto-yliopisto, Aalto University |
| سنة النشر: |
2019 |
| المجموعة: |
Aalto University Publication Archive (Aaltodoc) / Aalto-yliopiston julkaisuarkistoa |
| مصطلحات موضوعية: |
stirred tank bioreactor, oxygen mass transfer coefficient, power number, gas- exchange, fermentation, Escherichia coli |
| الوصف: |
Microorganisms are very tiny creatures that play an important part in the biotechnology industry. In certain environmental conditions, microbes metabolize and convert carbohydrates into alcohol or organic acids, a process known as fermentation. Different factors, such as temperature, air composition, nutrients and pH affects microbial growth. The purpose of this thesis was to study oxygen transfer behaviour and its influence on fermentation in a baffleless flask -fermentor-hybrid stirred-tank reactor (STR) (US patented Pat. 8,162,295) of Moubio Knowledge Co. The innovative design and portability of this tank make it a potentially important piece of laboratory equipment. The off-center impeller, the plastic vent tube and the thermometer well work together to create a possible mechanism for continuous gas exchange between the tank and the surrounding air. The tank (kLa = 29.57 h-1, 925 rpm) had 3 times oxygen transfer of Zoro’s [1] reactor (kLa = 10 h-1, 1000 rpm). In addition, this stirred tank reactor could grow microbes even at room temperature with 64 % of the same results as a shake flask incubating at an optimal temperature of 37 oC. In this project, Escherichia coli BL21 was used to produce green fluorescent protein (GFP) in the aerobic system. Oxygen transfer rate (OTR) was observed by iodometric titration, which was then used to calculate kLa values to form kLa correlations. OTR values changed based on the stirring speed, the depth of the plastic vent tube, and the depth of the thermometer well in the working volume. kLa correlation curves can be utilized in future tank design and gas-exchange research. ; Mikrobin kokoiset organismit ovat tärkeä osa biotekniikan teollisuudessa. Tietyissä ympäristön olosuhteissa mikrobit metaboloivat ja muuttavat hiilihydraatit alkoholeiksi ja orgaanisiksi hapoiksi, mitä kutsutaan fermentoinniksi. Erilaiset tekijät kuten lämpötila, ilma, ravinteet ja pH vaikuttavat mikrobikasvatukseen. Tutkimuksen tarkoituksena oli tarkastella hapen siirron käyttäytymistä ja ... |
| نوع الوثيقة: |
master thesis |
| اللغة: |
English |
| العلاقة: |
https://aaltodoc.aalto.fi/handle/123456789/37849Test; URN:NBN:fi:aalto-201905122951 |
| الإتاحة: |
https://aaltodoc.aalto.fi/handle/123456789/37849Test |
| رقم الانضمام: |
edsbas.3ED4115C |
| قاعدة البيانات: |
BASE |
