التفاصيل البيبلوغرافية
| العنوان: |
Life cycle assessment of mammalian cell culture medium with alternative compositions. ; Nisäkässolujen soluviljelyn ravinneliuoksen vaihtoehtoisten koostumusten elinkaariarviointi. |
| المؤلفون: |
Karinen, Heini |
| المساهمون: |
Tuomisto, Hanna, Insinööritieteiden korkeakoulu, Kummu, Matti, Aalto-yliopisto, Aalto University |
| سنة النشر: |
2023 |
| المجموعة: |
Aalto University Publication Archive (Aaltodoc) / Aalto-yliopiston julkaisuarkistoa |
| مصطلحات موضوعية: |
LCA, environmental impact, culture medium, protein hydrolysates, lignocellulosic glucose, openLCA |
| الوصف: |
Feasibility of the culture medium is one main factor affecting the industrial scalability and environmental impacts of cultivated meat. The commonly used culture medium includes ingredients that are expensive for mass production of cultivated meat, and especially use of fetal bovine serum (FBS) leads to ethical problems in addition to environmental impacts following from the beef cattle production. The main goal of the study was to compare the environmental impacts of the standard culture medium used in the mammalian cell culture to media with different variations on the composition and raw material sources. The first aim of the study was to assess the environmental impacts of protein hydrolysates used as a replacement of FBS in the culture medium. These include protein hydrolysates extracted from side-streams of livestock production (e.g., pork plasma and eggshell membranes) and plant-based protein hydrolysates, such as pea protein hydrolysate. Regarding the use of protein hydrolysates, consideration of cell growth (measured as DNA) during cell cultivation in the experiments conducted at Nofima was also included. Secondly, the environmental impacts of glucose production from alternative sources to maize and sugar cane (e.g., grains, straw, surplus grass, sawdust and forest chips) were assessed. Alternative raw material production was assumed to be located in Finland. The research was conducted following the ISO standard framework for life cycle assessment (LCA) using ReCiPe 2016 Hierarchist (H) midpoint method and Cumulative Energy Demand (CED) method. The system boundaries included the main processes from raw material extraction to ready-to-be-used culture medium. The functional unit of the study was one liter of culture medium. Product system was modelled with openLCA software. Agri-footprint 5.0 and Ecoinvent 3.7.1 databases were used for the LCA data. The data for the composition of the media were based on experiments carried out at Nofima. According to the results the environmental impacts can be ... |
| نوع الوثيقة: |
master thesis |
| وصف الملف: |
73 + 19; application/pdf |
| اللغة: |
English |
| العلاقة: |
https://aaltodoc.aalto.fi/handle/123456789/119382Test; URN:NBN:fi:aalto-202301291732 |
| الإتاحة: |
https://aaltodoc.aalto.fi/handle/123456789/119382Test |
| رقم الانضمام: |
edsbas.1E5663C9 |
| قاعدة البيانات: |
BASE |
