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1دورية أكاديمية
المؤلفون: Muhammad Hafiznur Yunus, Nor Azah Yusof, Suhainie Ismail, Siti Suraiya Md Noor, Faruq Mohammad, Yusran Sulaiman, Nurul Hanun Ahmad Raston, Jaafar Abdullah, Ahmed A. Soleiman
المصدر: Nanomaterials, Vol 12, Iss 15, p 2551 (2022)
مصطلحات موضوعية: tuberculosis detection, MPT64 antigen, aptamer, aptasensor, carboxyphenyl diazonium salt, sputum samples, Chemistry, QD1-999
الوصف: Curbing tuberculosis (TB) requires a combination of good strategies, including a proper prevention measure, diagnosis, and treatment. This study proposes an improvised tuberculosis diagnosis based on an amperometry approach for the sensitive detection of MPT64 antigen in clinical samples. An MPT64 aptamer specific to the target antigen was covalently attached to the carboxyphenyl diazonium-functionalized carbon electrode via carbodiimide chemistry. The electrochemical detection assay was adapted from a sandwich assay format to trap the antigen between the immobilized aptamer and horseradish peroxidase (HRP) tagged polyclonal anti-MPT64 antibody. The amperometric current was measured from the catalytic reaction response between HRP, hydrogen peroxide, and hydroquinone, which is used as an electron mediator. From the analysis, the detection limit in the measurement buffer was 1.11 ng mL−1. Additionally, the developed aptasensor exhibited a linear relationship between the current signal and the MPT64 antigen-spiked serum concentration ranging from 10 to 150 ng mL−1 with a 1.38 ng mL−1 detection limit. Finally, an evaluation using the clinical sputum samples from both TB (+) and TB (−) individuals revealed a sensitivity and specificity of 88% and 100%, respectively. Based on the analysis, the developed aptasensor was found to be simple in its fabrication, sensitive, and allowed for the efficient detection and diagnosis of TB in sputum samples.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Azger Dusthackeer, Magizhaveni Balasubramanian, Govindarajan Shanmugam, Shanmuga Priya, Christy Rosaline Nirmal, Rajadas Sam Ebenezer, Angayarkanni Balasubramanian, Rajesh Kumar Mondal, Kannan Thiruvenkadam, A. K. Hemanth Kumar, Geetha Ramachandran, Selvakumar Subbian
المصدر: Frontiers in Microbiology, Vol 10 (2019)
مصطلحات موضوعية: tuberculosis, bacteriological diagnosis, resuscitation, Mycobacteirum tuberculosis, clinical isolate organism, sputum samples, Microbiology, QR1-502
الوصف: Tuberculosis (TB) remains a leading killer among infectious diseases of humans worldwide. Delayed diagnosis is a crucial problem in global TB control programs. Bacteriological methods currently used to diagnose TB in endemic countries take up to 8 weeks, which poses a significant delay in starting antibiotic therapy. The presence of a heterogeneous population of Mycobacterium tuberculosis, the causative agent of TB, is among the reasons for delayed diagnosis by bacteriological methods. Previously, it has been shown that mycobacterial resuscitation-promoting factors (RPFs), a family of proteins secreted by actively growing bacteria into the media, are capable of activating the growth of dormant bacteria, thus enhancing the detection of bacilli in the sputum of confirmed TB cases. However, the variability in bacterial resuscitation by RPF in the sputum of suspected pulmonary TB cases that showed differential smear and/or culture positivity during diagnosis has not been fully explored. Here, we report the presence of non-replicating bacteria in the sputum of suspected TB cases that show differential growth response to RPF treatment. Using crude and recombinant RPF treatment, we show improved sensitivity and reduced time to detect bacilli in the sputum samples of smear-positive/culture-negative or smear-negative/culture-negative cases. We also report the phenotypic heterogeneity in the RPF responsiveness among Mtb strains using an in vitro dormancy model. Our findings have implications for improving the bacteriological diagnostic modalities currently used to diagnose TB in endemic countries.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/article/10.3389/fmicb.2019.02381/fullTest; https://doaj.org/toc/1664-302XTest
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