المؤلفون: Encinas, Micaela, Ferrara Muñiz, Ximena, Ayelén Sammarruco, Romina, Ruiz Menna, Victoria, Javier Garro, Carlos, Delgado, Fernando, Macías, Analía, Magnano, Gabriel, Zumárraga, Martín José, Garbaccio, Sergio Gabriel, Eirin, María Emilia

المصدر: Frontiers in Veterinary Science; 2024, p1-6, 6p

مصطلحات موضوعية: TUBERCULIN test, MYCOBACTERIUM bovis, SKIN tests, COW testing, TUBERCULOSIS in cattle

مستخلص: Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ...-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributable to Mycobacterium bovis (the causative agent of bTB) bacteraemia. A case--control study was conducted to investigate blood PCR as an alternative tool for improving ante-mortem detection of TST false-negative bovines. Cases comprised 70 TST false-negative bovines (cases), which were serology positive, and controls included 81 TST positive bovines; all of them confirmed as infected with M. bovis. Detection of the IS6110 target through touchdown blood-PCR (IS6110 TD-PCR) was performed. The positivity of the blood-PCR was 27.2% in the control group. This performance was similar to the 15% obtained among cases (p = 0.134). Most cases identified by the IS6110 TD-PCR exhibited focalized lesions (p = 0.002). Results demonstrated that blood-PCR could detect TST false-negative cattle, even if they are negative for IGRA. Considering that cases exhibited humoral response to M. bovis, further studies conducted in a pre-serological stage could provide evidence about the real contribution of the technique in herds. [ABSTRACT FROM AUTHOR]

: Copyright of Frontiers in Veterinary Science is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Marfil, María Jimena, Blanco, Federico Carlos, Colombatti Olivieri, María Alejandra, Eirin, María Emilia, Zumárraga, Martín José

المصدر: Frontiers in Cellular & Infection Microbiology; 2024, p01-07, 7p

مصطلحات موضوعية: MYCOBACTERIUM, MYCOBACTERIUM bovis, MYCOBACTERIUM tuberculosis, LUNG diseases, PINNIPEDIA, TUBERCULOSIS

مستخلص: The causative agent of tuberculosis in pinnipeds is Mycobacterium pinnipedii, a member of the Mycobacterium tuberculosis complex (MTC). The natural hosts are pinnipeds; however, other non-marine mammals, including humans, can also be infected. The transmissibility of a pathogen is related to its virulence. The transmissibility of a M. pinnipedii strain (i.e., 1856) was investigated in a murine model and compared with that of two Mycobacterium bovis strains (i.e., 534 and 04-303) with different reported virulence. Non-inoculated mice (sentinels) were co-housed with intratracheally inoculated mice. Detailed inspection of mice to search for visible tuberculosis lesions in the lungs and spleen was performed, and bacillus viability at 30, 60, and 90 days post-inoculation (dpi) was assayed. A transmissibility of 100% was recorded at 30 dpi in sentinel mice co-housed with the inoculated mice from the M. pinnipedii and M. bovis 04-303 groups, as evidenced by the recovery of viable M. pinnipedii and M. bovis from the lungs of sentinel mice. Mice inoculated with M. pinnipedii (1856) and M. bovis (534) survived until euthanized, whereas five of the M. bovis 04-303-inoculated mice died at 17 dpi. This study constitutes the first report of the transmissibility of a M. pinnipedii strain in mice and confirms the utility of this experimental model to study virulence features such as the transmission of poorly characterized MTC species. [ABSTRACT FROM AUTHOR]

: Copyright of Frontiers in Cellular & Infection Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Marfil, María Jimena, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Huertas, Pablo Sebastián, Vivot, Marcela Martínez, Eirin, María Emilia, Zumárraga, Martín José

المصدر: Foodborne Pathogens & Disease; Nov2021, Vol. 18 Issue 11, p805-811, 7p

المؤلفون: Marfil, María Jimena, Huertas, Pablo Sebastián, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Martínez Vivot, Marcela, Garro, Carlos, Alonso, Bernardo, Eirin, María Emilia, Zumárraga, Martín José

المصدر: Foodborne Pathogens & Disease; Dec2018, Vol. 15 Issue 12, p758-762, 5p