التفاصيل البيبلوغرافية
| العنوان: |
A PAX6-regulated receptor tyrosine kinase pairs with a pseudokinase to activate immune defense upon oomycete recognition in Caenorhabditis elegans. |
| المؤلفون: |
Drury, Florence1, Grover, Manish1, Hintze, Mark1, Saunders, Jonathan1, Fasseas, Michael K.1, Constantinou, Charis1, Barkoulas, Michalis1 m.barkoulas@imperial.ac.uk |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America. 9/26/2023, Vol. 120 Issue 39, p1-11. 26p. |
| مصطلحات موضوعية: |
*PROTEIN-tyrosine kinases, *CAENORHABDITIS elegans, *REGULATOR genes, *GENETIC testing, *CELL membranes |
| مستخلص: |
Oomycetes were recently discovered as natural pathogens of Caenorhabditis elegans, and pathogen recognition alone was shown to be sufficient to activate a protective transcriptional program characterized by the expression of multiple chitinase-like (chil) genes. However, the molecular mechanisms underlying oomycete recognition in animals remain fully unknown. We performed here a forward genetic screen to uncover regulators of chil gene induction and found several independent loss-of-function alleles of old-1 and flor-1, which encode receptor tyrosine kinases belonging to the C. elegans-specific KIN-16 family. We report that OLD-1 and FLOR-1 are both necessary for mounting the immune response and act in the epidermis. FLOR-1 is a pseudokinase that acts downstream of the active kinase OLD-1 and regulates OLD-1 levels at the plasma membrane. Interestingly, the old-1 locus is adjacent to the chil genes in the C. elegans genome, thereby revealing a genetic cluster important for oomycete resistance. Furthermore, we demonstrate that old-1 expression at the anterior side of the epidermis is regulated by the VAB-3/PAX6 transcription factor, well known for its role in visual system development in other animals. Taken together, our study reveals both conserved and species-specific factors shaping the activation and spatial characteristics of the immune response to oomycete recognition. [ABSTRACT FROM AUTHOR] |
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