التفاصيل البيبلوغرافية
| العنوان: |
The role of the platelet pool of Plasminogen Activator Inhibitor-1 in well-controlled type 2 diabetes patients |
| المؤلفون: |
Mossberg, Karin, 1981, Olausson, Josefin, 1983, Fryk, Emanuel, Jern, Sverker, 1954, Jansson, Per-Anders, 1961, Brogren, Helén, 1977 |
| المصدر: |
Plos One. 17(8 August) |
| مصطلحات موضوعية: |
Clinical Medicine, Klinisk medicin, messenger RNA, plasminogen activator inhibitor 1, plasminogen activator inhibitor 1 antigen, unclassified drug, plasminogen activator inhibitor 2, tissue plasminogen activator, adult, aged, antigen blood level, Article, cell level, clinical article, comparative study, controlled study, diabetes control, diabetic patient, enzyme linked immunosorbent assay, female, gene expression, human, human cell, human tissue, lean body weight, male, middle aged, non insulin dependent diabetes mellitus, obesity, polymerase chain reaction, thrombocyte, thrombocyte function, genetics, metabolism, Blood Platelets, Diabetes Mellitus, Type 2, Humans, RNA, Messenger |
| الوصف: |
Background The main inhibitor of the fibrinolytic system, Plasminogen Activator Inhibitor -1 (PAI-1), irreversibly binds tissue-type Plasminogen Activator (t-PA) and thereby inhibits the protective action of tPA against thrombus formation. Elevated levels of plasma PAI-1 are associated with an increased risk of cardiovascular events and are observed in subjects with type 2 diabetes (T2D) and obesity. Platelets contain the majority of PAI-1 present in blood and exhibit the ability to synthesis active PAI-1. Diabetic platelets are known to be hyper-reactive and larger in size; however, whether these features affect their contribution to the elevated levels of plasma PAI-1 in T2D is not established. Objectives To characterize the PAI-1 antigen content and the mRNA expression in platelets from T2D subjects compared to obese and lean control subjects, in order to elucidate the role of platelet PAI-1 in T2D. Methods Nine subjects with T2D and obesity were recruited from Primary Care Centers together with 15 healthy control subjects (8 lean subjects and 7 with obesity). PAI-1 antigen levels in plasma, serum and platelets were determined by ELISA, and PAI-1 mRNA expression was analyzed by qPCR. Results There was no significant difference in PAI-1 mRNA expression or PAI-1 antigen in platelets in T2D subject in comparison to obese and lean control subjects. An elevated level of plasma PAI-1 was seen in both T2D and obese subjects. PAI-1 gene expression was significantly higher in both obese groups compared to lean. Conclusion Similar levels of protein and mRNA expression of PAI-1 in platelets from T2D, obese and lean subjects indicate a limited role of platelets for the elevated plasma PAI-1 levels. However, an increased synthesis rate of mRNA transcripts in platelets from T2D and an increased release of PAI-1 could also result in similar mRNA and protein levels. Hence, synthesis and release rates of PAI-1 from platelets in T2D and obesity need to be investigated to further elucidate the role of platelets in obesity and T2D. © 2022 Mossberg et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| الوصول الحر: |
https://gup.ub.gu.se/publication/322519Test |
| قاعدة البيانات: |
SwePub |
