التفاصيل البيبلوغرافية
| العنوان: |
Cloning and functional analysis of CtDHN1 gene related to abiotic stress in Carthamus tinctorius. (English) |
| المؤلفون: |
DONG Cheng, YANG Jing-song, LIU Zi-yan, LI Hai-long, CHU Zhuo, WEN Qiu-ju, XIE Zi-yang, LIU Shu-ming, GUAN Li-li |
| المصدر: |
Chinese Journal of Oil Crop Sciences; 2020, Vol. 42 Issue 1, p85-90, 6p |
| مصطلحات موضوعية: |
ABIOTIC stress, SAFFLOWER, FUNCTIONAL analysis, LACTATE dehydrogenase, REVERSE transcriptase polymerase chain reaction, MOLECULAR weights, DROUGHT tolerance |
| مستخلص: |
Dehydrates was a member of the group 2 late embryogenesis abundant protein family, which were high disorder proteins and responded to adversity stress. Based on the transcriptome data of safflower under drought stress, CtDHN, a dehydrin gene respondimg to drought stress was identified and cloned by using RT-PCR techniques, which encoded 318 amino acids with the molecular weight of 31.1 kDa and the pI value of 6.36. Bioinformatics analysis indicated that CtDHN1 protein belonged to YnSKn-type dehydrin. Prokaryotic expression analysis revealed that CtDHN1 protein was a soluble protein. The protein had the highest expression level when the concentration of IPTG was 0.4 mmol/L, the OD600 value was 0.8, the induction time was 4 h, and the induction temperature was 37°C. The E. coli stress tolerance analysis demonstrated that CtDHN1 gene tolerated a drought and a high salt environment of 1.5 mol/L sorbitol and 1.3 mol/L sodium chloride. In vitro enzyme activity assays indicated that CtDHN1 protein protected LDH enzyme activity in 500 mmol/L and sorbitol 300 mmol/L sodium chloride. This research provided a theoretical basis for the molecular mechanism of CtDHN1 gene in safflower resistance to abiotic stress. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
