التفاصيل البيبلوغرافية
| العنوان: |
Histology-Specific MicroRNA Alterations in Melanoma. |
| المؤلفون: |
Poliseno, Laura1, Haimovic, Adele1, Segura, Miguel F2, Hanniford, Douglas2, Christos, Paul J3, Darvishian, Farbod4, Wang, Jinhua5, Shapiro, Richard L6, Pavlick, Anna C7, Berman, Russell S6, Hernando, Eva4, Zavadil, Jiri8, Osman, Iman9 |
| المصدر: |
Journal of Investigative Dermatology. Jul2012, Vol. 132 Issue 7, p1860-1868. 9p. |
| مصطلحات موضوعية: |
*HISTOLOGY, *NON-coding RNA, *MELANOMA, *GENOMICS, *CELL lines, *HEALTH outcome assessment, *GENE expression |
| مستخلص: |
We examined the microRNA signature that distinguishes the most common melanoma histological subtypes, superficial spreading melanoma (SSM) and nodular melanoma (NM). We also investigated the mechanisms underlying the differential expression of histology-specific microRNAs. MicroRNA array performed on a training cohort of 82 primary melanoma tumors (26 SSM, 56 NM), and nine congenital nevi (CN) revealed 134 microRNAs differentially expressed between SSM and NM (P<0.05). Out of 134 microRNAs, 126 remained significant after controlling for thickness and 31 were expressed at a lower level in SSM compared with both NM and CN. For seven microRNAs (let-7g, miR-15a, miR-16, miR-138, miR-181a, miR-191, and miR-933), the downregulation was associated with selective genomic loss in SSM cell lines and primary tumors, but not in NM cell lines and primary tumors. The lower expression level of six out of seven microRNAs in SSM compared with NM was confirmed by real-time PCR on a subset of cases in the training cohort and validated in an independent cohort of 97 melanoma cases (38 SSM, 59 NM). Our data support a molecular classification in which SSM and NM are two molecularly distinct phenotypes. Therapeutic strategies that take into account subtype-specific alterations might improve the outcome of melanoma patients. [ABSTRACT FROM AUTHOR] |
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