التفاصيل البيبلوغرافية
| العنوان: |
一种检测中药成分毒性的心脏芯片构建. (Chinese) |
| العنوان البديل: |
Construction of heart chip for evaluating toxicity of traditional Chinese medicine ingredients. (English) |
| المؤلفون: |
王 帅, 高 志 刚, 李 晓 瑞, 曲 玥 阳, 丛 烨, 张 索 楠, 罗 勇 |
| المصدر: |
Journal of Dalian University of Technology / Dalian Ligong Daxue Xuebao; Mar2022, Vol. 62 Issue 2, p158-164, 7p |
| مصطلحات موضوعية: |
CHINESE medicine, CULTURE media (Biology), POISONS, DRUG development, LACTATE dehydrogenase, CAPILLARIES |
| الملخص (بالإنجليزية): |
To evaluate the safety of traditional Chinese medicine (TCM), it is necessary to research on the dose effect toxicity relationships of TCM ingredients. Heart is an organ that may suffer from toxic effects and deserves attention in research in the development of new drugs. To solve the problems of lack of inter-cellular interactions and big differences between micro environment of well plates and real body, a heart chip that simulates the interaction between cardiomyocytes and capillaries in vitro is constructed to detect the toxicity of three TCM ingredients (triptolide, nitidine chloride and hyoscyamine). By comparing the changes of beating state of the cardiomyocytes on chip and well plate before and after the dosing, and the changes in the content of lactate dehydrogenase and troponin in the cell culture solution, it is found that the three chemical ingredients of TCM have dose-dependent cardiotoxicity. At the same time, it is found that the cardiomyocytes on the chip are more resistant to drugs than those on the well plate, this chip can be used to evaluate the cardiotoxicity of drugs. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
为确定中药安全性,有必要对中药中各成分的量效毒关系进行研究.心脏毒性是新药 研究中需要格外注意的器官毒性,为了解决孔板试验中缺少细胞间相互作用以及微环境与体 内差异大的问题,构建了一种在体外模拟心肌细胞与毛细血管相互作用的心脏芯片,并使用 该芯片检测了雷公藤甲素、氯化两面针碱、莨菪碱3种中药成分的毒性.通过对比加药前后芯 片和孔板里心肌细胞的搏动状态变化,以及细胞培养液中乳酸脱氢酶和肌钙蛋白的含量变 化,发现3种中药化学成分有剂量依赖的心脏毒性,同时发现芯片内的心肌细胞比孔板内的 对于药物的耐受性更强,显示了本芯片用于检测药物心脏毒性的潜力. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Dalian University of Technology / Dalian Ligong Daxue Xuebao is the property of Journal of Dalian University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
