التفاصيل البيبلوغرافية
| العنوان: |
Anticoagulants affect matrix metalloproteinase 9 levels in blood samples of stroke patients and healthy controls |
| المؤلفون: |
Rababah, Munir1 munir.rababa@googlemail.com, Worthmann, Hans1 worthmann.hans@mh-hannover.de, Deb, Milani1 deb.milani@mh-hannover.de, Tryc, Anita B.1 tryc.anita@mh-hannover.de, Ma, Yue Tao1 mayuetao@hotmail.com, El Bendary, Omnia M.2 obendary@yahoo.co.uk, Hecker, Hartmut3 hecker.hartmut@gmx.de, Goldbecker, Annemarie1 goldbecker.annemarie@mh-hannover.de, Heeren, Meike1 heeren.meike@mh-hannover.de, Brand, Korbinian2 brand.korbinian@mh-hannover.de, Weissenborn, Karin1 weissenborn.karin@mh-hannover.de, Lichtinghagen, Ralf2 lichtinghagen.ralf@mh-hannover.de |
| المصدر: |
Clinical Biochemistry. Apr2012, Vol. 45 Issue 6, p483-489. 7p. |
| مصطلحات موضوعية: |
*ANTICOAGULANTS, *METALLOPROTEINASES, *BLOOD sampling, *STROKE treatment, *LEUCOCYTES, *BIOMARKERS, *ENZYME-linked immunosorbent assay, *POLYMERASE chain reaction |
| مستخلص: |
Abstract: Objectives: Matrix metalloproteinase-9 (MMP-9) represents a promising marker for acute stroke management. In clinical studies MMP-9 has been quantified by ELISA using differing protocols. We aimed to establish a valid protocol by evaluation of preanalytics. Design and methods: Blood from stroke patients (n=28) and healthy controls (n=28) was drawn into tubes containing different anticoagulants (EDTA, citrate, lithium-heparin (heparin) and heparin with proteinase inhibitors) and processed after 0, 60 and 240min. MMP-9 plasma protein and mRNA from mononuclear leukocytes were determined. Results: In regard to anticoagulants used, samples showed different MMP-9 protein baseline values and kinetics. Stable MMP-9 protein concentrations were only measured from EDTA samples. Particularly in samples with proteinase inhibitors protein and mRNA concentrations increased over time. Kinetics did not differ between patients and controls. Conclusions: Preanalytics plays a key role for determination of MMP-9. EDTA seems to be a valid anticoagulant for MMP-9 protein measurement. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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