دورية أكاديمية

Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells

التفاصيل البيبلوغرافية
العنوان: Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells
المؤلفون: Chiara Vidoni, Alessandra Ferraresi, Eleonora Secomandi, Letizia Vallino, Chiara Gardin, Barbara Zavan, Carmen Mortellaro, Ciro Isidoro
المصدر: Cell Communication and Signaling, Vol 17, Iss 1, Pp 1-17 (2019)
بيانات النشر: BMC, 2019.
سنة النشر: 2019
المجموعة: LCC:Medicine
LCC:Cytology
مصطلحات موضوعية: AMPK, BECLIN-1, Phytotherapy, Osteoblast, Resveratrol, Medicine, Cytology, QH573-671
الوصف: Abstract Background/aim Autophagy is a macromolecular degradation process playing a pivotal role in the maintenance of stem-like features and in the morpho-functional remodeling of the tissues undergoing differentiation. In this work we investigated the involvement of autophagy in the osteogenic differentiation of mesenchymal stem cells originated from human gingiva (HGMSC). METHODS: To promote the osteogenic differentiation of HGMSCs we employed resveratrol, a nutraceutical known to modulate autophagy and cell differentiation, together with osteoblastic inductive factors. Osteoblastic differentiation and autophagy were monitored through western blotting and immunofluorescence staining of specific markers. Results We show that HGMSCs can differentiate into osteoblasts when cultured in the presence of appropriate factors and that resveratrol accelerates this process by up-regulating autophagy. The prolonged incubation with dexamethasone, β-glycerophosphate and ascorbic acid induced the osteogenic differentiation of HGMSCc with increased expression of autophagy markers. Resveratrol (1 μM) alone elicited a less marked osteogenic differentiation yet it greatly induced autophagy and, when added to the osteogenic differentiation factors, it provoked a synergistic effect. Resveratrol and osteogenic inductive factors synergistically induced the AMPK-BECLIN-1 pro-autophagic pathway in differentiating HGMSCs, that was thereafter downregulated in osteoblastic differentiated cells. Pharmacologic inhibition of BECLIN-1-dependent autophagy precluded the osteogenic differentiation of HGMSCs. Conclusions Autophagy modulation is instrumental for osteoblastic differentiation of HGMSCs. The present findings can be translated into the regenerative cell therapy of maxillary / mandibular bone defects. Graphical abstract
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1478-811X
العلاقة: http://link.springer.com/article/10.1186/s12964-019-0414-7Test; https://doaj.org/toc/1478-811XTest
DOI: 10.1186/s12964-019-0414-7
الوصول الحر: https://doaj.org/article/92849d4e956142eda06a77607b00d6a9Test
رقم الانضمام: edsdoj.92849d4e956142eda06a77607b00d6a9
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1478811X
DOI:10.1186/s12964-019-0414-7