التفاصيل البيبلوغرافية
| العنوان: |
Long non-coding RNAs link extracellularmatrix gene expression to ischemic cardiomyopathy. |
| المؤلفون: |
Zhan-Peng Huang, Yan Ding, Jinghai Chen, Gengze Wu, Masaharu Kataoka, Yongwu Hu, Jian-Hua Yang, Jianming Liu, Drakos, Stavros G., Selzman, Craig H., Kyselovic, Jan, Liang-Hu Qu, dos Remedios, Cristobal G., Pu, William T., Da-Zhi Wang |
| المصدر: |
Cardiovascular Research; 11/1/2016, Vol. 112 Issue 2, p543-554, 12p |
| مصطلحات موضوعية: |
NON-coding RNA, GENE expression, CORONARY disease, EXTRACELLULAR matrix proteins, RNA sequencing |
| مستخلص: |
Aims: Ischemic cardiomyopathy (ICM) resulting from myocardial infarction is a major cause of heart failure (HF). Recently, thousands of long non-coding RNAs (lncRNAs) have been discovered and implicated in a variety of biological processes. However, the role of most lncRNAs in HF remains largely unknown. The aim of this study is to test the hypothesis that the expression and function of lncRNAs are differentially regulated in diseased hearts. Methods and results: In this study, we performed RNA deep sequencing of protein-coding and non-coding RNAs from cardiac samples of patients with ICM (n?15) and controls (n=15). Genome-wide transcriptome analysis confirmed that many protein-coding genes previously known to be involved in HF were altered in ICM hearts. Among the 145 differentially expressed lncRNAs identified in ICM hearts, we found a set of 35 lncRNAs that display strong positive expression correlation. Expression correlation coefficient analyses of differentially expressed lncRNAs and proteincoding genes revealed a strong association between lncRNAs and extracellular matrix (ECM) protein-coding genes. We overexpressed or knocked down selected lncRNAs in cardiac fibroblasts and our results suggest that lncRNAs are important regulators of fibrosis and the expression of ECM synthesis genes. Moreover, we show that lncRNAs participate in the TGF-α pathway to modulate the expression of ECM genes and myofibroblast differentiation. Conclusion: Our studies demonstrate that the expression of many lncRNAs is dynamically regulated in ICM. lncRNAs regulate the expression and function of ECM and cardiac fibrosis during the development of ICM. Our results further indicate that lncRNAs may represent novel regulators of heart function and cardiac disorders, including ICM. [ABSTRACT FROM AUTHOR] |
|
Copyright of Cardiovascular Research is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder