دورية أكاديمية
Genome-wide expression assay comparison across frozen and fixed postmortem brain tissue samples
| العنوان: | Genome-wide expression assay comparison across frozen and fixed postmortem brain tissue samples |
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| المؤلفون: | Fu Xiang-Dong, Fan Jian-Bing, Wynshaw-Boris Anthony, Barnes Cynthia C, April Craig, Winn Mary E, Li Hai-Ri, Chow Maggie L, Courchesne Eric, Schork Nicholas J |
| المصدر: | BMC Genomics, Vol 12, Iss 1, p 449 (2011) |
| بيانات النشر: | BMC, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Biotechnology LCC:Genetics |
| مصطلحات موضوعية: | Brain, Gene Expression, DASL, IVT, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Abstract Background Gene expression assays have been shown to yield high quality genome-wide data from partially degraded RNA samples. However, these methods have not yet been applied to postmortem human brain tissue, despite their potential to overcome poor RNA quality and other technical limitations inherent in many assays. We compared cDNA-mediated annealing, selection, and ligation (DASL)- and in vitro transcription (IVT)-based genome-wide expression profiling assays on RNA samples from artificially degraded reference pools, frozen brain tissue, and formalin-fixed brain tissue. Results The DASL-based platform produced expression results of greater reliability than the IVT-based platform in artificially degraded reference brain RNA and RNA from frozen tissue-based samples. Although data associated with a small sample of formalin-fixed RNA samples were poor when obtained from both assays, the DASL-based platform exhibited greater reliability in a subset of probes and samples. Conclusions Our results suggest that the DASL-based gene expression-profiling platform may confer some advantages on mRNA assays of the brain over traditional IVT-based methods. We ultimately consider the implications of these results on investigations of neuropsychiatric disorders. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2164 |
| العلاقة: | http://www.biomedcentral.com/1471-2164/12/449Test; https://doaj.org/toc/1471-2164Test |
| DOI: | 10.1186/1471-2164-12-449 |
| الوصول الحر: | https://doaj.org/article/84f7b278bc6d4c5fbb3fb6372b6a16f2Test |
| رقم الانضمام: | edsdoj.84f7b278bc6d4c5fbb3fb6372b6a16f2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14712164 |
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| DOI: | 10.1186/1471-2164-12-449 |